Deapartment of Physical Chemistry, Universitat Rovira i Virgili, Carrer de Marcellí Domingo s/n, 43007, Tarragona, Spain.
Medcom Advance S.A., Av. Roma, 08840, Barcelona, Spain.
Angew Chem Int Ed Engl. 2017 Feb 20;56(9):2381-2385. doi: 10.1002/anie.201611243. Epub 2017 Jan 12.
Point mutations in Ras oncogenes are routinely screened for diagnostics and treatment of tumors (especially in colorectal cancer). Here, we develop an optical approach based on direct SERS coupled with chemometrics for the study of the specific conformations that single-point mutations impose on a relatively large fragment of the K-Ras gene (141 nucleobases). Results obtained offer the unambiguous classification of different mutations providing a potentially useful insight for diagnostics and treatment of cancer in a sensitive, fast, direct and inexpensive manner.
点突变的 Ras 癌基因是常规筛选的诊断和治疗肿瘤（尤其是在结直肠癌）。在这里，我们开发了一种基于直接 SERS 与化学计量学相结合的光学方法，用于研究单点突变对 K-Ras 基因（141 个核苷酸）相对较大片段施加的特定构象。所获得的结果提供了不同突变的明确分类，为癌症的诊断和治疗提供了一种敏感、快速、直接和廉价的潜在有用的方法。
