Tarangini Korumilli, Mishra Susmita
Department of Chemical Engineering, National Institute of Technology, Rourkela 769 008, Orissa, India.
Biotechnol Rep (Amst). 2014 Oct 7;4:139-146. doi: 10.1016/j.btre.2014.10.001. eCollection 2014 Dec.
In this study, optimization of production parameters influencing melanin production in an economical fruit waste extract was attempted using a garden soil isolate (). Taguchi approach was adopted for screening of critical parameters and further optimization was done using a central composite design of response surface methodology (RSM). At optimum conditions (pH 6.84 and Temp 30.7 °C), a significant yield of ∼6.96 mg/mL was observed. Statistical analysis revealed that the experimental results fitted well to the statistical model with model value 0.982. The optimization of process parameters using RSM reported a 15% increase in the pigment yield than average yield obtained from the studied model. The melanin produced was confirmed by UV-visible spectroscopy, FTIR and XRD analysis. Moreover melanin obtained has significant photoprotective, radical scavenging and metal chelating activity. Thus, has the potential to be a new source for the production of melanin, which is of industrial interest.
在本研究中，尝试使用一种从花园土壤中分离出的菌株，对影响经济实惠的水果废料提取物中黑色素生成的生产参数进行优化。采用田口方法筛选关键参数，并使用响应面法（RSM）的中心复合设计进行进一步优化。在最佳条件（pH 6.84和温度30.7°C）下，观察到黑色素产量显著达到约6.96 mg/mL。统计分析表明，实验结果与统计模型拟合良好，模型值为0.982。使用RSM对工艺参数进行优化后，色素产量比从研究模型获得的平均产量提高了15%。通过紫外可见光谱、傅里叶变换红外光谱（FTIR）和X射线衍射（XRD）分析对所产生的黑色素进行了确认。此外，所获得的黑色素具有显著的光保护、自由基清除和金属螯合活性。因此，该菌株有潜力成为黑色素生产的新来源，这具有工业价值。
