State Key Laboratory of Pulp and Paper Engineering and ‡State Key Laboratory of Luminescent Materials and Devices, South China University of Technology , Guangzhou 510640, P. R. China.
ACS Appl Mater Interfaces. 2017 Jul 12;9(27):22875-22884. doi: 10.1021/acsami.7b06168. Epub 2017 Jun 29.
A fluorescent "turn-on" sensor for fast measurement of aliphatic biogenic amines (BAs) was developed based on fluorescent carboxylated polyfluorene (PFTBTCOOH)/chitosan-graft-oleic acid (CS-graft-OA) micelles. In this system, biobased CS-graft-OA micelles, prepared by graft modification of CS with OA, serve as the vector of the hydrophobic fluorescent probe. Bright turn-on fluorescence was achieved by the intermolecular fluorescence resonance energy transfer of the encapsulated PFTBTCOOH, driven by the formation of an electrostatic complex with aliphatic BAs, which facilitate visual identification. The peak intensity ratio of the PFTBTCOOH/CS-graft-OA micelles toward an increasing amount of aliphatic BAs was calibrated, giving a linear relationship. The fluorescence response of PFTBTCOOH/CS-graft-OA micelles to aliphatic BAs in milk and yogurt matrixes reaches a detection concentration as low as 10 μM, showing that the PFTBTCOOH/CS-graft-OA micelle solution is a potential chemosensor for fast and selective detection of trace aliphatic BAs, which is of great significance to public health and food safety.
基于荧光羧基化聚芴（PFTBTCOOH）/壳聚糖接枝油酸（CS-graft-OA）胶束，开发了一种用于快速测量脂肪族生物胺（BAs）的荧光“开启”传感器。在该体系中，通过 OA 接枝改性 CS 制备的生物基 CS-graft-OA 胶束作为疏水性荧光探针的载体。通过与脂肪族 BAs 形成静电配合物，促进了包封的 PFTBTCOOH 的分子间荧光共振能量转移，实现了明亮的荧光“开启”，便于目视识别。通过校准 PFTBTCOOH/CS-graft-OA 胶束对越来越多的脂肪族 BAs 的峰值强度比，得出了线性关系。PFTBTCOOH/CS-graft-OA 胶束对牛奶和酸奶基质中脂肪族 BAs 的荧光响应达到低至 10 μM 的检测浓度，表明 PFTBTCOOH/CS-graft-OA 胶束溶液是一种用于快速选择性检测痕量脂肪族 BAs 的潜在化学传感器，这对公共健康和食品安全具有重要意义。
