Cpf1蛋白可从哺乳动物细胞的mRNA转录本中切除CRISPR RNA。
Cpf1 proteins excise CRISPR RNAs from mRNA transcripts in mammalian cells.
作者信息
Zhong Guocai, Wang Haimin, Li Yujun, Tran Mai H, Farzan Michael
机构信息
Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, Florida, USA.
Department of Microbiology and Wu Lien-Teh Institute, Harbin Medical University, Harbin, China.
出版信息
Nat Chem Biol. 2017 Aug;13(8):839-841. doi: 10.1038/nchembio.2410. Epub 2017 Jun 19.
Cpf1 is a CRISPR effector protein that has greater specificity than Streptococcus pyogenes Cas9 (SpCas9) in genome-editing applications. Here we show that Lachnospiraceae bacterium (Lb) and Acidaminococus sp. (As) Cpf1 orthologs have RNase activities that can excise multiple CRISPR RNAs (crRNAs) from a single RNA polymerase II-driven RNA transcript expressed in mammalian cells. This property simplifies modification of multiple genomic targets and can be used to increase the efficiency of Cpf1-mediated editing.
Cpf1是一种CRISPR效应蛋白，在基因组编辑应用中比化脓性链球菌Cas9（SpCas9）具有更高的特异性。我们在此表明，毛螺菌科细菌（Lb）和氨基酸球菌属（As）的Cpf1直系同源物具有核糖核酸酶活性，能够从在哺乳动物细胞中表达的单个RNA聚合酶II驱动的RNA转录本中切除多个CRISPR RNA（crRNA）。这一特性简化了对多个基因组靶点的修饰，可用于提高Cpf1介导的编辑效率。
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