Yao Hang, Xue Jingchen, Wang Qunfang, Xie Renjian, Li Weichang, Liu Sa, Cai Jinglei, Qin Dajiang, Wang Dong-An, Ren Li
School of Materials Science and Engineering, South China University of Technology, Wushan RD, Tianhe District, Guangzhou 510641, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China; Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, N1.3-B2-13, 637457, Singapore.
School of Materials Science and Engineering, South China University of Technology, Wushan RD, Tianhe District, Guangzhou 510641, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:661-670. doi: 10.1016/j.msec.2017.05.043. Epub 2017 May 11.
Glucosamine (GA) is an important cartilage matrix precursor for the glycosaminoglycan biochemical synthesis, and has positive effects on cartilage regeneration, particularly in osteoarthritis therapy. However, it has not been used as a bioactive group in scaffolds for cartilage repair widely. In this study, we synthesized modified polyethylene glycol (PEG) hydrogel with glucosamine and then encapsulated human bone mesenchymal stem cells (hBMSCs) in the hydrogel to induce the differentiation of hBMSCs into chondrocytes in three-dimensional culture. The GA-modified PEG hydrogels promoted the chondrogenesis of hBMSCs, particularly in the concentration of 5mM and 10mM. The subcutaneous transplantation of 10mM GA-modified hydrogels with hBMSCs formed cartilage-like blocks in vivo for 8weeks. Importantly, with glucosamine increase, the modified hydrogels down-regulated the fibrosis and hypertrophic cartilage markers in protein level. Therefore, glucosamine modified PEG hydrogels facilitated the chondrogenesis of hBMSCs, which might represent a new method for cartilage repair using a tissue-engineering approach.
氨基葡萄糖（GA）是糖胺聚糖生化合成的重要软骨基质前体，对软骨再生具有积极作用，尤其是在骨关节炎治疗中。然而，它尚未广泛用作软骨修复支架中的生物活性基团。在本研究中，我们合成了含氨基葡萄糖的改性聚乙二醇（PEG）水凝胶，然后将人骨髓间充质干细胞（hBMSCs）封装在水凝胶中，以在三维培养中诱导hBMSCs分化为软骨细胞。GA改性的PEG水凝胶促进了hBMSCs的软骨生成，尤其是在5mM和10mM的浓度下。皮下移植含10mM GA改性水凝胶的hBMSCs在体内8周形成了软骨样块。重要的是，随着氨基葡萄糖含量的增加，改性水凝胶在蛋白质水平上下调了纤维化和肥大软骨标志物。因此，氨基葡萄糖改性的PEG水凝胶促进了hBMSCs的软骨生成，这可能代表了一种使用组织工程方法进行软骨修复的新方法。
