Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea.
CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110007, India.
Bioresour Technol. 2017 Oct;241:922-927. doi: 10.1016/j.biortech.2017.05.160. Epub 2017 May 29.
Biohythane may be used as an alternative feed for methanol production instead of costly pure methane. In this study, methanol production potential of Methylocella tundrae immobilized through covalent immobilization, adsorption, and encapsulation was evaluated. Cells covalently immobilized on groundnut shells and chitosan showed a relative methanol production potential of 83.9 and 91.6%, respectively, compared to that of free cells. The maximum methanol production by free cells and cells covalently immobilized on groundnut shells and chitosan was 6.73, 6.20, and 7.23mM, respectively, using simulated biohythane as a feed. Under repeated batch conditions of eight cycles, cells covalently immobilized on chitosan and groundnut shells, and cells encapsulated in sodium-alginate resulted in significantly higher cumulative methanol production of 37.76, 31.80, and 25.58mM, respectively, than free cells (18.57mM). This is the first report on immobilization of methanotrophs on groundnut shells and its application in methanol production using biohythane as a feed.
生物甲烷可以替代昂贵的纯甲烷作为甲醇生产的替代原料。本研究评估了通过共价固定化、吸附和包封固定化的嗜甲基菌（Methylocella tundrae）生产甲醇的潜力。与游离细胞相比，固定在落花生壳和壳聚糖上的细胞的相对甲醇生产潜力分别为 83.9%和 91.6%。利用模拟生物甲烷作为进料，游离细胞和固定在落花生壳和壳聚糖上的细胞的最大甲醇产量分别为 6.73、6.20 和 7.23mM。在 8 个循环的重复分批条件下，固定在壳聚糖和落花生壳上的细胞以及包埋在海藻酸钠中的细胞的累积甲醇产量分别显著高于游离细胞（18.57mM），达到 37.76、31.80 和 25.58mM。这是首次报道将花生壳用于甲烷营养菌的固定化及其在以生物甲烷为原料生产甲醇中的应用。
