体外不同增殖潜能的人成纤维细胞通过收缩胶原晶格产生类组织结构。
Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro.
作者信息
Bell E, Ivarsson B, Merrill C
出版信息
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1274-8. doi: 10.1073/pnas.76.3.1274.
Abstract
Fibroblasts can condense a hydrated collagen lattice to a tissue-like structure 1/28th the area of the starting gel in 24 hr. The rate of the process can be regulated by varying the protein content of the lattice, the cell number, or the concentration of an inhibitor such as Colcemid. Fibroblasts of high population doubling level propagated in vitro, which have left the cell cycle, can carry out the contraction at least as efficiently as cycling cells. The potential uses of the system as an immunologically tolerated "tissue" for wound healing and as a model for studying fibroblast function are discussed.
摘要
成纤维细胞可在24小时内将水合胶原晶格浓缩成一种组织样结构，其面积为起始凝胶面积的1/28。该过程的速率可通过改变晶格中的蛋白质含量、细胞数量或抑制剂（如秋水仙酰胺）的浓度来调节。在体外传代培养至较高群体倍增水平且已离开细胞周期的成纤维细胞，其收缩效率至少与处于细胞周期的细胞相同。本文还讨论了该系统作为用于伤口愈合的免疫耐受“组织”以及作为研究成纤维细胞功能模型的潜在用途。
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