Kämpfer Peter, Busse Hans-Jürgen, McInroy John A, Glaeser Stefanie P
Institut für Angewandte Mikrobiologie, Universität Giessen, Germany.
Institut für Mikrobiologie, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Int J Syst Evol Microbiol. 2017 Jun;67(6):1740-1745. doi: 10.1099/ijsem.0.001852. Epub 2017 Jun 20.
A slightly yellow-pigmented, Gram-stain-negative, rod-shaped bacterium, strain IMT-305T, was isolated from soil in Alabama, USA. Phylogenetic analysis based on the nearly full-length 16S rRNA gene sequence placed the strain in between the genera Pusillimonas, Parapusillimonas and Candidimonas with highest 16S rRNA gene sequence similarity to the type strain of Parapusillimonas granuli (97.5 %) and Candidimonas nitroreducens (97.4 %). The genomic G+C content of strain IMT-305T was 63.9 mol%. The main cellular fatty acids were C18:1ω7c, C17:0 cyclo, C16:0 and C16:1ω7c/iso-C15:0 2-OH (detected as summed feature 3). The polyamine pattern of strain IMT-305T contained the major compound putrescine and the betaproteobacterial diagnostic 2-hydroxyputrescine and the major respiratory quinone was ubiquinone Q-8. Predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, an unidentified aminolipid, an unidentified aminophospholipid and an unidentified lipid lacking any functional group. Based on phylogenetic, chemotaxonomic and phenotypic analyses a novel species within a new genus, Paracandidimonas soli gen. nov., sp. nov., is proposed. The type strain of Paracandidimonas soli is IMT-305T (=DSM 100048T=CIP 110902T=LMG 28740T=CCM 8599T).
从美国阿拉巴马州的土壤中分离出一株略带黄色色素、革兰氏染色阴性的杆状细菌，菌株IMT-305T。基于近乎全长的16S rRNA基因序列的系统发育分析将该菌株置于Pusillimonas属、Parapusillimonas属和Candidimonas属之间，其16S rRNA基因序列与Parapusillimonas granuli模式菌株（97.5%）和Candidimonas nitroreducens模式菌株（97.4%）的相似度最高。菌株IMT-305T的基因组G+C含量为63.9 mol%。主要细胞脂肪酸为C18:1ω7c、C17:0环丙烷、C16:0和C16:1ω7c/iso-C15:0 2-OH（检测为总和特征3）。菌株IMT-305T的多胺模式包含主要化合物腐胺和β-变形菌诊断性的2-羟基腐胺，主要呼吸醌为泛醌Q-8。主要极性脂质为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰丝氨酸、一种未鉴定的氨基脂质、一种未鉴定的氨基磷脂和一种缺乏任何官能团的未鉴定脂质。基于系统发育、化学分类和表型分析，提出了一个新属Paracandidimonas内的一个新物种，Paracandidimonas soli gen. nov., sp. nov.。Paracandidimonas soli的模式菌株为IMT-305T（=DSM 100048T=CIP 110902T=LMG 28740T=CCM 8599T）。
