Ge Chunmei, Lu Weiqun, Chen Aqin
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education (Shanghai Ocean University), China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education (Shanghai Ocean University), China.
Biochem Biophys Res Commun. 2017 Aug 26;490(3):657-663. doi: 10.1016/j.bbrc.2017.06.093. Epub 2017 Jun 17.
The molecular mechanisms underlying final oocyte maturation in zebrafish (Danio rerio) remain poorly understood. The present study aimed to employ iTRAQ approach for a comprehensive characterization of during zebrafish oocyte maturation proteome and for comparison between fully-grow immature and mature oocytes prior to ovulation. A total of 1568 proteins were identified, which was representing the largest zebrafish isolated oocytes proteome dataset to date. Differential expression analysis revealed 190 proteins significantly changes between immature and mature oocytes, which 136 proteins were up-regulated and 54 proteins were down-regulated in mature oocytes comparison with immature oocytes. Functional analysis revealed that these differential proteins were mostly involved in cellular response to estrogen stimulus, cellular components, extracellular region, and enzyme regulator activity, etc. The revealed differentially changes in protein expression patterns associated with oocyte maturation suggest that several of the examined proteins, such as vitellogenin(Vtg3), protein S100(S100A10), 17-beta hydroxysteroid dehydrogenase(HSD17B1), pentaxin, zona pellucida (ZP3.2), elongation factor1-alpha, caluemnin B, and 14-3-3 protein may play a specific role during zebrafish final oocyte maturation. These data will provide powerful information for understanding the molecular mechanism underlying zebrafish oocyte maturation, and these proteins may potentially act as markers to predict control oocyte maturation of zebrafish oocytes.
斑马鱼（Danio rerio）最终卵母细胞成熟的分子机制仍知之甚少。本研究旨在采用iTRAQ方法全面表征斑马鱼卵母细胞成熟过程中的蛋白质组，并比较排卵前完全成熟的未成熟卵母细胞和成熟卵母细胞。共鉴定出1568种蛋白质，这是迄今为止最大的斑马鱼分离卵母细胞蛋白质组数据集。差异表达分析显示，未成熟卵母细胞和成熟卵母细胞之间有190种蛋白质发生了显著变化，与未成熟卵母细胞相比，成熟卵母细胞中有136种蛋白质上调，54种蛋白质下调。功能分析表明，这些差异蛋白质大多参与细胞对雌激素刺激的反应、细胞成分、细胞外区域和酶调节活性等。所揭示的与卵母细胞成熟相关的蛋白质表达模式的差异变化表明，一些检测到的蛋白质，如卵黄蛋白原（Vtg3）、蛋白S100（S100A10）、17-β羟基类固醇脱氢酶（HSD17B1）、五聚素、透明带（ZP3.2）、延伸因子1-α、钙调蛋白B和14-3-3蛋白可能在斑马鱼最终卵母细胞成熟过程中发挥特定作用。这些数据将为理解斑马鱼卵母细胞成熟的分子机制提供有力信息，这些蛋白质可能潜在地作为预测和控制斑马鱼卵母细胞成熟的标志物。
