Chan Yin-Ching, Hwang Juen-Haur
Department of Food and Nutrition, Providence University, Taichung, Taiwan.
Department of Otolaryngology, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Chiayi, Taiwan.
PLoS One. 2017 Jun 21;12(6):e0178916. doi: 10.1371/journal.pone.0178916. eCollection 2017.
To our knowledge, the effects of Spirulina platensis water extract (SP) on hearing function have not yet been reported. This study investigated the effects of SP on the function and redox status of the auditory system. Auditory brainstem responses and redox status were compared between two groups of 3-month-old senescence-accelerated prone-8 (SAMP8) mice: the control group was fed a normal diet, and the experimental group was fed a normal diet with oral supplementation of SP for 6 weeks. Compared with the control group, the experimental group had significantly lower hearing thresholds according to auditory brainstem responses measured using click sounds and 8-kHz tone burst sound stimulation at the end of this study. The experimental group had a shorter I-III interval of auditory brainstem responses with 16-kHz tone burst stimulation than the control group that was of borderline significance. Additionally, the experimental group had significantly higher mRNA expression of the superoxide dismutase and catalase genes in the cochlea and brainstem and significantly higher mRNA expression of the glutathione peroxidase gene in the cochlea. Further, the experimental group had significantly lower malondialdehyde levels in the cochlea and brainstem than the control group. However, tumor necrosis factor-α mRNA expression was not significantly different between the control and experimental groups. SP could decrease hearing degeneration in senescence-accelerated prone-8 mice possibly by increasing superoxide dismutase, catalase, and glutathione peroxidase gene expression and decreasing damage from oxidative stress in the cochlea and brainstem.
据我们所知，钝顶螺旋藻水提取物（SP）对听力功能的影响尚未见报道。本研究调查了SP对听觉系统功能和氧化还原状态的影响。比较了两组3月龄快速老化小鼠8（SAMP8）的听觉脑干反应和氧化还原状态：对照组给予正常饮食，实验组在正常饮食基础上口服补充SP 6周。在本研究结束时，与对照组相比，实验组根据使用短声和8kHz短纯音刺激测量的听觉脑干反应，听力阈值显著降低。在16kHz短纯音刺激下，实验组听觉脑干反应的I-III间期比对照组短，具有临界显著性。此外，实验组耳蜗和脑干中超氧化物歧化酶和过氧化氢酶基因的mRNA表达显著更高，耳蜗中谷胱甘肽过氧化物酶基因的mRNA表达也显著更高。此外，实验组耳蜗和脑干中的丙二醛水平显著低于对照组。然而，对照组和实验组之间肿瘤坏死因子-α的mRNA表达没有显著差异。SP可能通过增加超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶基因表达以及减少耳蜗和脑干中氧化应激的损伤来减少快速老化小鼠8的听力退化。
