Health Care Department Changzhou Maternity and Child Health Care Hospital Affiliated with Nanjing Medical University Changzhou China.
Department of Rehabilitation No. 102 Hospital of Chinese People's Liberation Army Changzhou China.
Brain Behav. 2017 May 2;7(6):e00711. doi: 10.1002/brb3.711. eCollection 2017 Jun.
WHO stated that nearly one million people commit suicide every year worldly, and 40% of the suicide completer suffered from depression. The primary aim of this study was to explore the association between long noncoding RNAs (lncRNAs) expression in peripheral blood mononuclear cells (PBMCs) and suicide risk of patients with major depressive disorder (MDD).
Using Human LncRNA 3.0 microarray profiling which includes 30,586 human lncRNAs and RT-PCR, six down-regulated lncRNAs were identified differentially expressed in MDD patients. According to suicidal ideation and suicidal attempt, the suicide risk of MDD patients was classified into suicidal ideation versus no suicidal ideation groups, and past attempt versus no past attempt groups, respectively. The expression of six lncRNAs in MDD patients and controls were examined by RT-PCR.
The expression of six lncRNAs had significant differences between no suicidal ideation, suicidal ideation, and controls; corresponding lncRNAs associated with suicidal attempt had remarkable differences between no past attempt, past attempt, and controls. Additionally, only the expression of lncRNAs in suicidal ideation group and past attempt group markedly declined compared with controls.
This study indicated that the expression of six down-regulated lncRNAs had a negative association with suicide risk in MDD patients, and the expression of lncRNAs in PBMCs could have the potential to help clinician judge the suicide risk of MDD patients to provide timely treatment and prevent suicide.
世界卫生组织表示，每年全球有近 100 万人自杀，其中 40%的自杀者患有抑郁症。本研究的主要目的是探讨外周血单个核细胞（PBMCs）中长链非编码 RNA（lncRNAs）表达与重度抑郁症（MDD）患者自杀风险之间的关系。
采用 Human LncRNA 3.0 微阵列分析，其中包含 30586 个人类 lncRNAs 和 RT-PCR，鉴定出 MDD 患者中 6 个下调的 lncRNAs 表达差异。根据自杀意念和自杀企图，将 MDD 患者的自杀风险分为有自杀意念与无自杀意念组，以及有自杀企图与无自杀企图组。采用 RT-PCR 检测 MDD 患者和对照组中 6 个 lncRNAs 的表达。
无自杀意念、有自杀意念和对照组之间，有 6 个 lncRNAs 的表达存在显著差异；与自杀企图相关的相应 lncRNAs 在无过去企图、过去企图和对照组之间也存在显著差异。此外，只有自杀意念组和过去企图组的 lncRNAs 表达明显低于对照组。
本研究表明，6 个下调的 lncRNAs 的表达与 MDD 患者的自杀风险呈负相关，PBMCs 中 lncRNAs 的表达可能有助于临床医生判断 MDD 患者的自杀风险，以便及时治疗和预防自杀。
