Wang Yong Gui, Jiang Liang Bo, Gou Bo
Department of Orthopaedics, Xiangyang No.1 People's Hospital affiliated to Hubei University of Medicine, Xiangyang, Hubei 441000, China.
Department of Microscopic Orthopaedics, Shiyan People's Hospital affiliated to Hubei University of Medicine, Shiyan, Hubei 442000, China.
Afr J Tradit Complement Altern Med. 2017 Jun 5;14(4):31-38. doi: 10.21010/ajtcam.v14i4.4. eCollection 2017.
The need for an anti-osteoporotic agent is in high demand since osteoporosis contributes to high rates of disability or impairment (high osteoporotic fracture), morbidity and mortality. Hence, the present study is designed to evaluate the protective effects of vanillic acid (VA) against bilateral ovariectomy-induced osteoporosis in female Sprague-Dawley (SD) rats.
Forty healthy female adult SD rats were separated in to four groups with sham-operated control with bilateral laprotomy (Sham; = 10), bilateral overiectomy (OVX; = 10) group, OVX rats were orallay administrated with 50 mg/kg b.wt of VA (OVX + 50 VA; = 10) or 100 mg/kg b.wt of VA (OVX + 100 VA; = 10) for 12 weeks (post-treatment) after 4 weeks of OVX.
A significant change in the body weight gain was noted in OVX group, while treatment with VA substantially reverted to normalcy. Meanwhile, the bone mineral density and content (BMD and BMC) were substantially improved on supplementation with VA. Also, the bone turnover markers like calcium (Ca), phosphorous (P), osteocalcin (OC), alkaline phosphatase (ALP) and deoxypyridinoline (DPD) and inflammatory markers (IL-1β, IL-6, and TNF-α) levels were markedly attenuated in VA-treated rats. Moreover, the biomechanical stability was greatly ameliorated with VA administration. Both the dose of VA showed potent anti-osteoporotic activity, but VA 100 mg showed highest protective effects as compared with 50 mg of VA.
Based on the outcome, we concluded that VA 100 showed better anti-osteoporotic activity by improving BMD and BMC as well as biomechanical stability and therefore used as an alternative therapy for treating postmenopausal osteoporosis.
由于骨质疏松症导致高致残率或功能障碍（高骨质疏松性骨折）、发病率和死亡率，对抗骨质疏松药物的需求很高。因此，本研究旨在评估香草酸（VA）对雌性Sprague-Dawley（SD）大鼠双侧卵巢切除诱导的骨质疏松症的保护作用。
40只健康成年雌性SD大鼠分为四组，双侧剖腹手术假手术对照组（假手术组；n = 10）、双侧卵巢切除组（OVX组；n = 10），OVX大鼠在卵巢切除4周后口服给予50 mg/kg体重的VA（OVX + 50 VA组；n = 10）或100 mg/kg体重的VA（OVX + 100 VA组；n = 10），共12周（治疗后）。
OVX组体重增加有显著变化，而VA治疗可使其基本恢复正常。同时，补充VA后骨矿物质密度和含量（BMD和BMC）显著改善。此外，VA治疗的大鼠骨转换标志物如钙（Ca）、磷（P）、骨钙素（OC）、碱性磷酸酶（ALP）和脱氧吡啶啉（DPD）以及炎症标志物（IL-1β、IL-6和TNF-α）水平明显降低。此外，VA给药可大大改善生物力学稳定性。两种剂量的VA均表现出有效的抗骨质疏松活性，但与50 mg VA相比，100 mg VA显示出最高的保护作用。
基于结果，我们得出结论，100 mg VA通过改善BMD和BMC以及生物力学稳定性表现出更好的抗骨质疏松活性，因此可作为治疗绝经后骨质疏松症的替代疗法。
