Busula A O, Takken W, DE Boer J G, Mukabana W R, Verhulst N O
Laboratory of Entomology, Wageningen University and Research, Wageningen, the Netherlands.
International Centre of Insect Physiology and Ecology, Nairobi, Kenya.
Med Vet Entomol. 2017 Sep;31(3):320-326. doi: 10.1111/mve.12242. Epub 2017 Jun 22.
The host preferences of the anthropophilic mosquito species in the Anopheles gambiae complex (Diptera: Culicidae) are mediated by skin bacterial volatiles. However, it is not known whether these mosquitoes respond differentially to skin bacterial volatiles from non-human host species. In this study, the responses of two malaria mosquito species in the An. gambiae complex, Anopheles gambiae s.s. (hereafter, An. gambiae) and Anopheles arabiensis, with different host preferences, to volatiles released from skin bacteria were tested. Skin bacteria collected from human, cow and chicken skin significantly increased trap catches; traps containing bacteria collected from human skin caught the highest proportions of An. gambiae and An. arabiensis. Traps with bacteria of human origin caught a significantly higher proportion of An. gambiae than of An. arabiensis, whereas bacterial volatiles from the chicken attracted significantly higher numbers of An. arabiensis than of An. gambiae. Additionally, An. gambiae showed a specialized response to volatiles from four specific bacteria, whereas An. arabiensis responded equally to all species of bacteria tested. Skin bacterial volatiles may therefore play important roles in guiding mosquitoes with different host preferences. The identification of these bacterial volatiles can contribute to the development of an odour blend that attracts mosquitoes with different host preferences.
冈比亚按蚊复合体（双翅目：蚊科）中嗜人血蚊种的宿主偏好由皮肤细菌挥发物介导。然而，尚不清楚这些蚊子对非人类宿主物种的皮肤细菌挥发物是否有不同反应。在本研究中，测试了冈比亚按蚊复合体中两种具有不同宿主偏好的疟蚊，即冈比亚按蚊指名亚种（以下简称冈比亚按蚊）和阿拉伯按蚊，对皮肤细菌释放的挥发物的反应。从人、牛和鸡皮肤上收集的皮肤细菌显著增加了诱捕器捕获量；装有从人皮肤上收集的细菌的诱捕器捕获的冈比亚按蚊和阿拉伯按蚊比例最高。含有源自人类细菌的诱捕器捕获的冈比亚按蚊比例显著高于阿拉伯按蚊，而来自鸡的细菌挥发物吸引的阿拉伯按蚊数量显著多于冈比亚按蚊。此外，冈比亚按蚊对四种特定细菌的挥发物表现出特异性反应，而阿拉伯按蚊对所有测试的细菌种类反应相同。因此，皮肤细菌挥发物可能在引导具有不同宿主偏好的蚊子方面发挥重要作用。鉴定这些细菌挥发物有助于开发一种气味混合物，吸引具有不同宿主偏好的蚊子。
