MTego 陷阱：监测疟疾和虫媒病毒媒介的潜在工具。

The MTego trap: a potential tool for monitoring malaria and arbovirus vectors.

机构信息

Vector Control Product Testing Unit, Environmental Health and Ecological Science Department, Ifakara Health Institute, P.O. Box 74, Bagamoyo, Tanzania.

School of Life Sciences and Bioengineering, The Nelson Mandela African Institution of Science and Technology (NM-AIST), P.O. Box 447, Arusha, Tanzania.

出版信息

Parasit Vectors. 2023 Jun 27;16(1):212. doi: 10.1186/s13071-023-05835-9.

DOI:10.1186/s13071-023-05835-9

PMID:37370169

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10294468/

Abstract

BACKGROUND

Odour-baited traps are useful for vector surveillance and control. However, most existing traps have shown inconsistent recapture rates across different mosquito species, necessitating the need for more effective and efficient traps. The MTego trap with integrated thermal stimuli has been developed as an alternative trap. This study was undertaken to determine and compare the efficacy of the MTego trap to that of the Biogents (BG) modular BG-Pro (BGP) trap for sampling different mosquito species in a semi-field system.

METHODS

Fully balanced Latin square design experiments (no-choice and dual choice) were conducted in semi-field chambers using laboratory-reared female Anopheles gambiae sensu stricto, Anopheles funestus, Anopheles arabiensis, Culex quinquefasciatus and Aedes aegypti. There were 16 replicates, and 50 mosquitoes of each species were released in each chamber per replicate. The evaluated traps were as follows: the MTego trap baited with PM6 (MT-PM6), the MTego trap baited with BG-Lure (BGL) (MT-BGL), and the BGP trap baited with BG-Lure (BGP-BGL).

RESULTS

In the no-choice test, the MT-BGL and BGP-BGL traps captured a similar proportion of An. gambiae (31% vs 29%, P-value = 0.519) and An. funestus (32% vs 33%, P = 0.520). The MT-PM6 and BGP-BGL traps showed no significant difference in capturing Ae. aegypti (33% vs 31%, P = 0.324). However, the BGP-BGL caught more An. arabiensis and Cx. quinquefasciatus mosquitoes than the other traps (P < 0.0001). In the dual-choice test of MT-PM6 vs BGP-BGL, similar proportions of An. funestus (25% vs 27%, P = 0.473) and Ae. aegypti (29% vs 25%, P = 0.264) were captured in the traps, while the BGP-BGL captured more An. gambiae, An. arabiensis and Cx. quinquefasciatus mosquitoes than the MT-PM6 (P < 0.0001).

CONCLUSIONS

This study demonstrated that the MTego trap has potential as a tool that can be used interchangeably with the BGP trap for sampling anthropophilic mosquitoes including African malaria vectors An. gambiae and An. funestus and the principal arbovirus vector Ae. aegypti.

摘要

背景

气味诱捕器在病媒监测和控制方面非常有用。然而，大多数现有的诱捕器在不同蚊种的重捕率上存在不一致，因此需要更有效和高效的诱捕器。集成热刺激的 MTego 诱捕器已被开发为替代诱捕器。本研究旨在确定和比较 MTego 诱捕器与 Biogents（BG）模块化 BG-Pro（BGP）诱捕器在半野外系统中对不同蚊种的采样效果。

方法

在半野外室内使用实验室饲养的雌性冈比亚按蚊、致倦库蚊、阿拉伯按蚊、淡色库蚊和埃及伊蚊进行完全平衡拉丁方设计实验（无选择和双重选择）。每个实验有 16 个重复，每个重复中有 50 只蚊子。评估的诱捕器如下：用 PM6 诱饵的 MTego 诱捕器（MT-PM6）、用 BG-Lure 诱饵的 MTego 诱捕器（MT-BGL）和用 BG-Lure 诱饵的 BGP 诱捕器（BGP-BGL）。

结果

在无选择测试中，MT-BGL 和 BGP-BGL 诱捕器捕获的冈比亚按蚊和致倦库蚊比例相似（分别为 31%和 29%，P 值=0.519）。MT-PM6 和 BGP-BGL 诱捕器捕获埃及伊蚊的比例无显著差异（分别为 33%和 31%，P=0.324）。然而，BGP-BGL 诱捕到的阿拉伯按蚊和淡色库蚊比其他诱捕器多（P<0.0001）。在 MT-PM6 与 BGP-BGL 的双重选择测试中，诱捕器捕获的致倦库蚊和埃及伊蚊比例相似（分别为 25%和 27%，P=0.473），而 BGP-BGL 诱捕到的冈比亚按蚊、阿拉伯按蚊和淡色库蚊比 MT-PM6 诱捕器多（P<0.0001）。