Linder J, Cassling R S, Rogler W C, Wilson J E, Markin R S, Sears T D, McManus B M
Arch Pathol Lab Med. 1985 Oct;109(10):917-20.
Evaluation of endomyocardial biopsy specimens for lymphocytic myocarditis requires accurate identification of lymphocytes, a task at times difficult considering that other myocardial interstitial cells mimic lymphocyte morphology. To wit, the number of mononuclear cells present in normal (uninflamed) myocardium has remained in doubt. We studied the myocardium from hearts that were obtained at autopsy and transvenous endomyocardial biopsy specimens with monoclonal antibodies and immunohistochemical stains to determine the normal numbers and distribution of lymphocytes in uninflamed hearts. In the ventricular myocardium of hearts obtained at autopsy, total immunohistochemically marked lymphocytes averaged 3.6/sq mm, with most being T-cell marker-positive. The ratio of T-helper to T-suppressor-cytotoxic (OKT-4:OKT-8) cells was 1.44. The number of myocardial lymphocytes demonstrated by immunohistochemical staining correlated well with, but was consistently less than, the number obtained by quantitative light microscopic studies on unmarked samples. Thus, the immunohistochemical technique allows for objective enumeration of cells and provides avenues for quantitation of lymphocyte subpopulations in inflamed hearts.
对心内膜心肌活检标本进行淋巴细胞性心肌炎评估时，需要准确识别淋巴细胞，鉴于其他心肌间质细胞会模仿淋巴细胞形态，这一任务有时颇具难度。也就是说，正常（未发炎）心肌中存在的单核细胞数量一直存在疑问。我们使用单克隆抗体和免疫组织化学染色法，研究了尸检获取的心脏心肌以及经静脉心内膜心肌活检标本，以确定未发炎心脏中淋巴细胞的正常数量和分布情况。在尸检获取的心脏心室肌中，免疫组织化学标记的淋巴细胞总数平均为每平方毫米3.6个，其中大多数为T细胞标记阳性。辅助性T细胞与抑制性/细胞毒性T细胞（OKT-4:OKT-8）的比例为1.44。免疫组织化学染色显示的心肌淋巴细胞数量与对未标记样本进行定量光学显微镜研究所得数量密切相关，但始终少于后者。因此，免疫组织化学技术能够对细胞进行客观计数，并为定量分析发炎心脏中的淋巴细胞亚群提供途径。
