Madsen Mikael, Christensen Rasmus S, Krissanaprasit Abhichart, Bakke Mette R, Riber Camilla F, Nielsen Karina S, Zelikin Alexander N, Gothelf Kurt V
Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000, Aarhus C, Denmark.
Present address: Department of Materials Science and Engineering, North Carolina State University, Raleigh, North Carolina, 27606, USA.
Chemistry. 2017 Aug 4;23(44):10511-10515. doi: 10.1002/chem.201702780. Epub 2017 Jul 17.
Conjugated polymers have been intensively studied due to their unique optical and electronic properties combined with their physical flexibility and scalable bottom up synthesis. Although the bulk qualities of conjugated polymers have been extensively utilized in research and industry, the ability to handle and manipulate conjugated polymers at the nanoscale lacks significantly behind. Here, the toolbox for controlled manipulation of conjugated polymers was expanded through the synthesis of a polyfluorene-DNA graft-type polymer (poly(F-DNA)). The polymer possesses the characteristics associated with the conjugated polyfluorene backbone, but the protruding single-stranded DNA provides the material with an exceptional addressability. This study demonstrates controlled single-molecule patterning of poly(F-DNA), as well as energy transfer between two different polymer-DNA conjugates. Finally, highly efficient DNA-directed quenching of polyfluorene fluorescence was shown.
由于共轭聚合物具有独特的光学和电子特性，同时具备物理柔韧性和可扩展的自下而上合成方法，因此受到了广泛研究。尽管共轭聚合物的整体性质已在研究和工业中得到广泛应用，但在纳米尺度上处理和操控共轭聚合物的能力仍存在显著不足。在此，通过合成聚芴 - DNA接枝型聚合物（聚（F - DNA）），扩展了用于可控操控共轭聚合物的工具箱。该聚合物具有与共轭聚芴主链相关的特性，但突出的单链DNA赋予了该材料特殊的可寻址性。本研究展示了聚（F - DNA）的可控单分子图案化，以及两种不同聚合物 - DNA共轭物之间的能量转移。最后，还展示了聚芴荧光的高效DNA定向猝灭。
