Laredo-Naranjo Martha Alicia, Carrillo-Gonzalez Roberto, De La Garza-Ramos Myriam Angelica, Garza-Navarro Marco Antonio, Torre-Martinez Hilda H H, Del Angel-Mosqueda Casiano, Mercado-Hernandez Roberto, Carrillo-Fuentevilla Roberto
Posgrado de Ortodoncia, Facultad de Odontologia, Universidad Autonoma de Nuevo LeónMonterreyMexico.
Centro de Investigacion y Desarrollo en Ciencias de la Salud, Universidad Autonoma de Nuevo LeonMonterreyMexico.
Acta Biomater Odontol Scand. 2016 Mar 29;2(1):60-67. doi: 10.3109/23337931.2016.1160783. eCollection 2016 Dec.
To evaluate the antimicrobial properties and dental pulp stem cells (DPSCs) cytotoxicity of synthesized carboxymethyl cellulose-silver nanoparticles impregnated on titanium plates. The antibacterial effect of silver nanoparticles in a carboxymethyl cellulose matrix impregnated on titanium plates (Ti-AgNPs) in three concentrations: 16%, 50% and 100% was determined by adding these to bacterial cultures of and . The Ti-AgNPs cytotoxicity on DPSCs was determined using a fluorimetric cytotoxicity assay with 0.12% chlorhexidine as a positive control. Silver nanoparticles in all concentrations were antimicrobial, with concentrations of 50% and 100% being more cytotoxic with 4% cell viability. Silver nanoparticles 16% had a cell viability of 95%, being less cytotoxic than 0.12% chlorhexidine. Silver nanoparticles are a promising structure because of their antimicrobial properties. These have high cell viability at a concentration of 16%, and are less toxic than chlorhexidine.
评估负载于钛板上的合成羧甲基纤维素-银纳米颗粒的抗菌性能及对牙髓干细胞（DPSC）的细胞毒性。通过将三种浓度（16%、50%和100%）负载于钛板上的羧甲基纤维素基质中的银纳米颗粒（Ti-AgNPs）添加到 和 的细菌培养物中，测定其抗菌效果。使用以0.12%洗必泰作为阳性对照的荧光细胞毒性测定法，测定Ti-AgNPs对DPSC的细胞毒性。所有浓度的银纳米颗粒均具有抗菌性，50%和100%浓度的银纳米颗粒细胞毒性更强，细胞活力为4%。16%浓度的银纳米颗粒细胞活力为95%，细胞毒性低于0.12%的洗必泰。银纳米颗粒因其抗菌性能而成为一种有前景的结构。它们在16%的浓度下具有较高的细胞活力，且毒性低于洗必泰。
