Maastricht MultiModal Molecular Imaging (M4I) institute, Division of Imaging Mass Spectrometry, Maastricht University, Universiteitssingel 50, 6229 ER, Maastricht, The Netherlands.
Analyst. 2017 Jul 24;142(15):2690-2712. doi: 10.1039/c7an00565b.
Mass spectrometry is being used in many clinical research areas ranging from toxicology to personalized medicine. Of all the mass spectrometry techniques, mass spectrometry imaging (MSI), in particular, has continuously grown towards clinical acceptance. Significant technological and methodological improvements have contributed to enhance the performance of MSI recently, pushing the limits of throughput, spatial resolution, and sensitivity. This has stimulated the spread of MSI usage across various biomedical research areas such as oncology, neurological disorders, cardiology, and rheumatology, just to name a few. After highlighting the latest major developments and applications touching all aspects of translational research (i.e. from early pre-clinical to clinical research), we will discuss the present challenges in translational research performed with MSI: data management and analysis, molecular coverage and identification capabilities, and finally, reproducibility across multiple research centers, which is the largest remaining obstacle in moving MSI towards clinical routine.
质谱分析在许多临床研究领域都有应用，从毒理学到个性化医疗。在所有的质谱分析技术中，质谱成像（MSI），尤其是，不断向临床应用方向发展。重大的技术和方法学的改进有助于提高 MSI 的性能，提高了通量、空间分辨率和灵敏度。这刺激了 MSI 在包括肿瘤学、神经紊乱、心脏病学和风湿病学等多个生物医学研究领域的应用。在强调了最新的主要发展和应用，涉及转化研究的各个方面（即从早期临床前研究到临床研究）之后，我们将讨论在使用 MSI 进行转化研究中目前面临的挑战：数据管理和分析、分子覆盖和鉴定能力，以及最后，在多个研究中心之间的可重复性，这是将 MSI 推向临床常规的最大遗留障碍。
