用改进的相量法和拉盖尔反卷积进行快速荧光寿命估计：比较研究。
Rapid fluorescence lifetime estimation with modified phasor approach and Laguerre deconvolution: a comparative study.
机构信息
Department of Pathology and Laboratory Medicine, 4400 V Street, CA 95817, United States of America.
出版信息
Methods Appl Fluoresc. 2017 Sep 1;5(3):035003. doi: 10.1088/2050-6120/aa7b62.
Abstract
Fluorescence lifetime imaging has been shown to serve as a valuable tool for interrogating and diagnosis of biological tissue at a mesoscopic level. The ability to analyze fluorescence decay curves to extract lifetime values in real-time is crucial for clinical translation and applications such as tumor margin delineation or intracoronary imaging of atherosclerotic plaques. In this work, we compare the performance of two popular non-parametric (fit-free) methods for determining lifetime values from fluorescence decays in real-time-the Phasor approach and Laguerre deconvolution. We demonstrate results from simulated and experimental data to compare the accuracy and speed of both methods and their dependence on noise and model parameters.
摘要
荧光寿命成像是一种在介观水平上研究和诊断生物组织的有价值的工具。实时分析荧光衰减曲线以提取寿命值的能力对于临床转化和应用至关重要，例如肿瘤边界描绘或动脉粥样硬化斑块的冠状动脉内成像。在这项工作中，我们比较了两种流行的非参数（无拟合）方法从实时荧光衰减中确定寿命值的性能 - 相位向量方法和拉盖尔反卷积。我们展示了模拟和实验数据的结果，以比较两种方法的准确性和速度及其对噪声和模型参数的依赖性。
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