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DNA结合蛋白的电学探针

Electrical Probes of DNA-Binding Proteins.

作者信息

Barton Jacqueline K, Bartels Phillip L, Deng Yingxin, O'Brien Elizabeth

机构信息

California Institute of Technology, Pasadena, CA, United States.

California Institute of Technology, Pasadena, CA, United States.

出版信息

Methods Enzymol. 2017;591:355-414. doi: 10.1016/bs.mie.2017.03.024. Epub 2017 May 10.

DOI:10.1016/bs.mie.2017.03.024
PMID:28645377
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6314295/
Abstract

A DNA electrochemistry platform has been developed to probe proteins bound to DNA electrically. Here gold electrodes are modified with thiol-modified DNA, and DNA charge transport chemistry is used to probe DNA binding and enzymatic reaction both with redox-silent and redox-active proteins. For redox-active proteins, the electrochemistry permits the determination of redox potentials in the DNA-bound form, where comparisons to DNA-free potentials can be made using graphite electrodes without DNA modification. Importantly, electrochemistry on the DNA-modified electrodes facilitates reaction under aqueous, physiological conditions with a sensitive electrical measurement of binding and activity.

摘要

一种用于电探测与DNA结合的蛋白质的DNA电化学平台已被开发出来。在此，金电极用硫醇修饰的DNA进行修饰，并且DNA电荷传输化学被用于探测与氧化还原沉默和氧化还原活性蛋白质的DNA结合及酶促反应。对于氧化还原活性蛋白质，电化学能够测定其以DNA结合形式存在时的氧化还原电位，在此可使用未修饰DNA的石墨电极将其与游离DNA的电位进行比较。重要的是，在DNA修饰电极上进行的电化学操作有利于在水性生理条件下进行反应，并能对结合和活性进行灵敏的电学测量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af1a/6314295/c7f527acaddd/nihms-999521-f0009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af1a/6314295/c7f527acaddd/nihms-999521-f0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af1a/6314295/ae57243b3f13/nihms-999521-f0001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af1a/6314295/367edda1cea0/nihms-999521-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af1a/6314295/95173a6dba19/nihms-999521-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af1a/6314295/49f267fe1f56/nihms-999521-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af1a/6314295/51e885ad9a3e/nihms-999521-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af1a/6314295/669c9dfe9246/nihms-999521-f0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af1a/6314295/c7f527acaddd/nihms-999521-f0009.jpg

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本文引用的文献

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Long-Range Electron Transfer through DNA Films.通过DNA薄膜的长程电子转移。
Angew Chem Int Ed Engl. 1999 Apr 1;38(7):941-945. doi: 10.1002/(SICI)1521-3773(19990401)38:7<941::AID-ANIE941>3.0.CO;2-7.
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DNA-mediated signaling by proteins with 4Fe-4S clusters is necessary for genomic integrity.具有4个铁-4个硫簇的蛋白质通过DNA介导的信号传导对于基因组完整性是必需的。
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