Bello Bello Aminu, Khan Shahid Ali, Khan Jalaluddin Awllia, Syed Fareeduddin Quadri, Anwar Yasir, Khan Sher Bahadar
Department of Biochemistry, Faculty of Science, King Abdul-Aziz University, Jeddah, Saudi Arabia; Department of Biochemistry, Federal University Dutse, P.M.B., 7156 Dutse, Jigawa State, Nigeria.
Center of Excellence for Advanced Materials Research (CEAMR), King Abdul-Aziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia.
J Photochem Photobiol B. 2017 Oct;175:99-108. doi: 10.1016/j.jphotobiol.2017.07.031. Epub 2017 Aug 19.
The study concentrate on the biosynthesis of silver nanoparticles (AgNps) from the leaves extract of Guiera senegalensis with focus on its; antiproliferation effect on prostate (PC3), breast (MCF7) and liver (HepG2) cancer cell lines, antibacterial effect on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) and the degradation on 4-nitrophenol (4-NP) and congo red dye (CR). The synthesized AgNps were characterized by FTIR, TEM, FESEM, XRD and EDX analysis. The EDS spectrum revealed that the synthesized nanoparticles (Nps) were composed of 55.45% Ag atoms of spherical shape with approximately 50nm size, identified from TEM and FESEM data. The antiproliferation effect of the AgNps varies with cell lines in a concentration dependent manner. The result showed that the AgNps were more effective on PC3 (IC 23.48μg/mL) than MCF7 (29.25μg/mL) and HepG2 (33.25μg/mL) by the virtue of their IC values. The AgNps were highly effective against E. coli and S. aureus by killing 99% colonies. The AgNps also shows a good catalytic reduction of the toxic organic pollutants in which only 3mg of the AgNps degraded 95% of both CR dye and 4-NP in 22 and 36min respectively. Therefore, the green synthesis of AgNps may have potential applications in pharmacology and industries for the treatment of cancers, bacterial infections and in degrading toxic organic pollutants in water.
该研究聚焦于从塞内加尔盖拉的叶片提取物中生物合成银纳米颗粒（AgNps），重点研究其对前列腺癌细胞系（PC3）、乳腺癌细胞系（MCF7）和肝癌细胞系（HepG2）的抗增殖作用，对大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）的抗菌作用，以及对4-硝基苯酚（4-NP）和刚果红染料（CR）的降解作用。通过傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）、场发射扫描电子显微镜（FESEM）、X射线衍射（XRD）和能谱分析（EDX）对合成的AgNps进行了表征。能谱分析表明，合成的纳米颗粒（Nps）由55.45%的银原子组成，呈球形，尺寸约为50nm，这是根据TEM和FESEM数据确定的。AgNps的抗增殖作用因细胞系而异，且呈浓度依赖性。结果表明，根据半数抑制浓度（IC）值，AgNps对PC3（IC为23.48μg/mL）的效果比对MCF7（29.25μg/mL）和HepG2（33.25μg/mL）更显著。AgNps对大肠杆菌和金黄色葡萄球菌具有高效抗菌作用，可杀死99%的菌落。AgNps还对有毒有机污染物表现出良好的催化还原作用，仅3mg的AgNps分别在22分钟和36分钟内就降解了95%的CR染料和4-NP。因此，AgNps的绿色合成在癌症治疗、细菌感染治疗以及降解水中有毒有机污染物的药理学和工业领域可能具有潜在应用。
