Perveen Irum, Raza Muhammad Asam, Iqbal Tahir, Naz Iffat, Sehar Shama, Ahmed Safia
Microbiology Research Laboratory, Department of Microbiology, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Department of Chemistry, University of Gujrat, Gujrat, Pakistan.
Microb Pathog. 2017 Sep;110:214-224. doi: 10.1016/j.micpath.2017.06.033. Epub 2017 Jun 23.
Owing to the importance of endophytes, current research was aimed to purify the secondary metabolites from targeted source. Ferula sumbul, a lipophilic extract of the endophyte was prepared in 10% methanol and partitioned with ethyl acetate and bioassay guided isolation was carried using standard protocols against bacterial, fungal and cancer cells. The active fractions consisted of three new metabolites (2-methyl-3-nonyl prodiginine, Bis (2-ethylhexyl) phthalate, and a meroterpenoid, Preaustinoid A). Their structures were confirmed with LCMS/MS. The purified metabolites showed valuable results against tested activities which concluded that these compounds have great potential and these may be applicable to textile (dyeing), pharmaceutical (drug, infectious agents) and food (preservatives) industries. This study reveals the potential of E. nigrum as an important source of bioactive compounds including 2-methyl-3-nonyl prodiginine, Bis (2-ethylhexyl) phthalate, and Preaustinoid A. This is first report of isolation of prodiginines as well as meroterpenoid and Bis (2-ethylhexyl) phthalate from Epicoccum nigrum.
由于内生菌的重要性，当前的研究旨在从目标来源中纯化次生代谢产物。将内生菌的亲脂性提取物阿魏（Ferula sumbul）用10%甲醇制备，并与乙酸乙酯进行分配，然后按照标准方案对细菌、真菌和癌细胞进行生物测定导向分离。活性部分包含三种新的代谢产物（2-甲基-3-壬基灵菌红素、邻苯二甲酸二（2-乙基己基）酯和一种杂萜类化合物，前奥司汀A）。通过液相色谱-质谱联用/质谱（LCMS/MS）确定了它们的结构。纯化后的代谢产物在测试活性方面显示出有价值的结果，这表明这些化合物具有很大的潜力，可能适用于纺织（染色）、制药（药物、传染病原体）和食品（防腐剂）行业。本研究揭示了黑附球菌（E. nigrum）作为包括2-甲基-3-壬基灵菌红素、邻苯二甲酸二（2-乙基己基）酯和前奥司汀A在内的生物活性化合物重要来源的潜力。这是首次从黑附球菌中分离出灵菌红素以及杂萜类化合物和邻苯二甲酸二（2-乙基己基）酯的报道。
