Zhang Ziwei, Liu Qi, Cai Jingzeng, Yang Jie, Shen Qiang, Xu Shiwen
Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Department of Clinical Cancer Prevention, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
Fish Shellfish Immunol. 2017 Aug;67:604-611. doi: 10.1016/j.fsi.2017.06.048. Epub 2017 Jun 22.
Chlorpyrifos (CPF) is an environmental pollutant with increasing importance due to its high toxicity to fish and aquatic animals. To understand how CPF impacts immune response and oxidative stress in common carp (Cyprinus carpio L.), we investigated the transcriptomic profiles of the head kidneys from common carp exposed to CPF for 15days. A total of 52, 297, 928 and 52, 273, 784 high quality clean reads were obtained from CPF exposure groups, 44,677,578 and 44,106,696 high quality clean reads were obtained from corresponding control groups. Among them, 456 genes were differentially expressed, including 246 up-regulated genes and 210 down-regulated genes identified and enriched in databases of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Antioxidant systems, and immune response genes and pathways were verified by quantitative real-time RT-PCR. We found that CPF-induced ROS regulates immune response by stimulating the antigen-presenting ability of head kidney in carp. These results provide new insights for unveiling the biological effects of CPF in common carp.
毒死蜱（CPF）是一种环境污染物，因其对鱼类和水生动物具有高毒性而日益受到关注。为了解CPF如何影响鲤鱼（Cyprinus carpio L.）的免疫反应和氧化应激，我们研究了暴露于CPF 15天的鲤鱼头部肾脏的转录组图谱。CPF暴露组分别获得了52297928和52273784条高质量的 clean reads，相应对照组分别获得了44677578和44106696条高质量的 clean reads。其中，456个基因差异表达，包括246个上调基因和210个下调基因，这些基因在基因本体论（GO）和京都基因与基因组百科全书（KEGG）数据库中被鉴定和富集。通过定量实时RT-PCR验证了抗氧化系统以及免疫反应基因和途径。我们发现CPF诱导的ROS通过刺激鲤鱼头部肾脏的抗原呈递能力来调节免疫反应。这些结果为揭示CPF对鲤鱼的生物学效应提供了新的见解。
