Department of Biomedical Engineering, School of Medicine, Tsinghua University , Beijing 100084, China.
ACS Appl Mater Interfaces. 2017 Jul 19;9(28):23528-23535. doi: 10.1021/acsami.7b06814. Epub 2017 Jul 5.
Glucose oxidase (GOX) can convert glucose into gluconic acid and hydrogen peroxide (HO), which is potentially useful for synergistic cancer-starving and oxidation therapy. Herein we demonstrate a glucose-responsive nanomedicine made of GOX-polymer nanogels to regulate HO production for synergistic melanoma starving and oxidation therapy. GOX-polymer nanogels showed glucose-responsive HO-generating activity in vitro, improved stability, and considerably enhanced tumor retention as compared to native GOX. More importantly, they exhibited high antimelanoma efficacy and no obvious systemic toxicity, whereas native GOX was ineffective and systemically toxic at the same dose. This work paves the way for establishing an endogenous and noninvasive cancer treatment paradigm that is based on intratumoral glucose-responsive, HO-generating chemical reactions.
葡萄糖氧化酶 (GOX) 可以将葡萄糖转化为葡萄糖酸和过氧化氢 (HO)，这对于协同饥饿和氧化治疗癌症具有潜在的应用价值。在此，我们展示了一种由 GOX-聚合物纳米凝胶制成的葡萄糖响应型纳米药物，用于调节 HO 的产生，以实现协同黑色素饥饿和氧化治疗。与天然 GOX 相比，GOX-聚合物纳米凝胶在体外表现出葡萄糖响应的 HO 生成活性、提高的稳定性和显著增强的肿瘤保留能力。更重要的是，它们表现出了很高的抗黑色素瘤功效，而且没有明显的全身毒性，而相同剂量的天然 GOX 则无效且具有全身毒性。这项工作为建立一种基于肿瘤内葡萄糖响应、HO 生成的化学反应的内源性、非侵入性癌症治疗范式铺平了道路。
