Shao Fenjuan, Lu Qiang, Wilson Iain W, Qiu Deyou
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, The Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.
CSIRO Agriculture & Food, PO Box 1600, Canberra, ACT 2601, Australia.
Gene. 2017 Sep 5;627:315-321. doi: 10.1016/j.gene.2017.06.044. Epub 2017 Jun 23.
SQUAMOSA Promoter-Binding Protein-Likes (SPLs) are plant specific transcription factors playing important roles in plant growth and development. The SPL gene family has been studied in various plant species; however, there is no report about SPLs in Zizyphus jujuba. In this study, we identified 18 putative ZjSPL genes in Z. jujuba using a genome-wide analysis. Sequence features, gene structures, conserved domains and motifs were analyzed. The phylogenetic relationships of SPLs in Z. jujuba and A. thaliana were revealed. A total of 5 pairs of ZjSPLs were identified, suggesting the importance of gene duplication in SPL gene expansion in Z. jujuba. In addition, 11 of the 18 ZjSPLs, belonging to G1, G2 and G5 subgroups, were found to be targets of miR156, suggesting the conservation of miR156-mediated posttranscriptional regulation in plants. Expression analysis revealed that eight ZjSPL genes were responsive to the infection of witches'-broom phytoplasma. Our results provide a basis for the further elucidation of the biological function of ZjSPLs and their regulation in witches'-broom disease.
SQUAMOSA启动子结合蛋白类似物（SPLs）是植物特有的转录因子，在植物生长发育中发挥着重要作用。SPL基因家族已在多种植物物种中得到研究；然而，关于枣（Zizyphus jujuba）中SPLs的研究尚无报道。在本研究中，我们通过全基因组分析在枣中鉴定出18个假定的ZjSPL基因。对其序列特征、基因结构、保守结构域和基序进行了分析。揭示了枣和拟南芥中SPLs的系统发育关系。共鉴定出5对ZjSPLs，表明基因复制在枣SPL基因扩展中的重要性。此外，18个ZjSPLs中的11个属于G1、G2和G5亚组，被发现是miR156的靶标，这表明植物中miR156介导的转录后调控具有保守性。表达分析表明，8个ZjSPL基因对枣疯病植原体感染有响应。我们的结果为进一步阐明ZjSPLs的生物学功能及其在枣疯病中的调控提供了依据。
