Cohen Joshua L, Barile Daniela, Liu Yan, de Moura Bell Juliana M L N
Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA, 95616, United States.
Foods for Health Institute, University of California, Davis, One Shields Avenue, Davis, CA, 95616, United States.
Int Dairy J. 2017 Mar;66:68-75. doi: 10.1016/j.idairyj.2016.10.016. Epub 2016 Nov 21.
Milk oligosaccharides are associated with improved health outcomes in infants. Nanofiltration (NF) is used for isolation of bovine milk oligosaccharides (BMO). The study aim was to improve the recovery of BMO from lactose-hydrolyzed colostrum whey permeate. The retention factors of carbohydrates at various pH and transmembrane pressures were determined for a nanofiltration membrane, which was used at pilot scale to purify BMO. Carbohydrates were quantified by liquid chromatography and characterized using nano-LC-Chip-QToF mass spectrometry. BMO purity was improved from an initial 4% in colostrum whey permeate to 98%, with 99.8% permeation of monosaccharides and 96% recovery of oligosaccharides, represented by 23 unique BMO compounds identified in the final retentate. The pH during NF was a determining factor in the selectivity of carbohydrate separation. This NF method can be applied to conventional cheese-whey permeate and other milk types for extraction of bioactive oligosaccharides providing new options for the dairy industry.
乳寡糖与婴儿更好的健康状况相关。纳滤（NF）用于分离牛乳寡糖（BMO）。本研究的目的是提高从乳糖水解初乳清渗透物中回收BMO的效率。测定了纳滤膜在不同pH值和跨膜压力下碳水化合物的保留因子，该纳滤膜在中试规模下用于纯化BMO。通过液相色谱对碳水化合物进行定量，并使用纳升液相色谱-芯片-四极杆飞行时间质谱对其进行表征。BMO的纯度从初乳清渗透物中的初始4%提高到了98%，单糖的渗透率为99.8%，寡糖的回收率为96%，最终截留物中鉴定出23种独特的BMO化合物。纳滤过程中的pH值是碳水化合物分离选择性的决定性因素。这种纳滤方法可应用于传统奶酪乳清渗透物和其他类型的牛奶，以提取生物活性寡糖，为乳制品行业提供了新的选择。
