Translational Musculoskeletal Research Center, Corporal Michael J. Crescenz Philadelphia VA Medical Center, 3900 University & Woodland Avenue, Philadelphia, PA, 19104, USA.
McKay Orthopaedic Research Lab, University of Pennsylvania, 424 Stemmler Hall, 36th Street & Hamilton Walk, Philadelphia, PA, 19104, USA.
Ann Biomed Eng. 2017 Oct;45(10):2348-2359. doi: 10.1007/s10439-017-1876-7. Epub 2017 Jun 26.
While delayed delivery of non-steroidal anti-inflammatory drugs (NSAIDs) has been associated with improved tendon healing, early delivery has been associated with impaired healing. Therefore, NSAID use is appropriate only if the dose, timing, and mode of delivery relieves pain but does not impede tissue repair. Because delivery parameters can be controlled using drug-eluting nanofibrous scaffolds, our objective was to develop a scaffold for local controlled release of ibuprofen (IBP), and characterize the release profile and degradation both in vitro and in vivo. We found that when incubated in vitro in saline, scaffolds containing IBP had a linear release profile. However, when implanted subcutaneously in vivo or when incubated in vitro in serum, scaffolds showed a rapid burst release. These data demonstrate that scaffold properties are dependent on the environment in which they are placed and the importance of using serum, rather than saline, for initial in vitro evaluation of biofactor release from biodegradable scaffolds.
虽然非甾体抗炎药（NSAIDs）的延迟递送与改善肌腱愈合有关，但早期递送与愈合受损有关。因此，只有在剂量、时间和给药方式缓解疼痛而不阻碍组织修复的情况下，才适合使用 NSAID。由于可以使用载药纳米纤维支架来控制给药参数，我们的目标是开发一种用于局部控释布洛芬（IBP）的支架，并对其在体外和体内的释放曲线和降解情况进行表征。我们发现，当在盐水中体外孵育时，含有 IBP 的支架具有线性释放曲线。然而，当皮下植入体内或在血清中体外孵育时，支架会迅速释放。这些数据表明，支架的性质取决于其所处的环境，以及在最初使用血清而非盐水评估可生物降解支架中生物因子释放时的重要性。
