Ezzat-Zadeh Zahra, Kim Jeong-Su, Chase P Bryant, Arjmandi Bahram H
Department of Nutrition, Food, and Exercise Sciences, Florida State University, Tallahassee, FL, USA.
Center for Advancing Exercise and Nutrition Research on Aging (CAENRA), College of Human Sciences, Florida State University, Tallahassee, FL 32306, USA.
J Aging Res. 2017;2017:1454103. doi: 10.1155/2017/1454103. Epub 2017 Jun 1.
Obesity, osteoporosis, and sarcopenia may individually occur due to age-related gradual alterations in body composition. This study investigates the cooccurrence of these age-related diseases in female animals with low levels of ovarian hormone in the absence of complex multifactorial process of chronological aging.
Thirty-six 5- and 10-month-old female rats were chosen to model pre- and postmenopausal women, respectively. Rats were divided into three treatment groups in each age category-sham, ovariectomized (ovx), and ovx + E (17-estradiol, 10 g/kg)-and were pair-fed. Volunteer wheel running activity, body composition, bone microstructure, serum C-telopeptides of type I collagen, bone specific alkaline phosphatase, E, and gastrocnemius and soleus muscles were analyzed.
The cooccurrence of osteoporosis, sarcopenia, and obesity was observed in the older ovx rats associated with a significant ( < 0.05) increased fat mass (30%), bone loss (9.6%), decreased normalized muscle mass-to-body-weight ratio (10.5%), and a significant decrease in physical activity (57%). The ratio of tibial bone mineral density to combined muscle mass was significantly decreased in both ovx age categories.
Ovariectomized rat could be used as an experimental model to examine the effect of loss of ovarian hormones, while controlling for energy intake and expenditure, to conduct obesity and body composition translational research in females without the confounding effect of genetic background.
肥胖、骨质疏松症和肌肉减少症可能因与年龄相关的身体成分逐渐变化而单独出现。本研究调查了在没有复杂多因素自然衰老过程的情况下，这些与年龄相关的疾病在卵巢激素水平低的雌性动物中的共现情况。
分别选择36只5个月和10个月大的雌性大鼠作为绝经前和绝经后女性的模型。将每个年龄组的大鼠分为三个治疗组——假手术组、卵巢切除（ovx）组和ovx + E（17-β-雌二醇，10μg/kg）组——并进行配对喂养。分析了自愿轮转跑步活动、身体成分、骨微结构、血清I型胶原C-末端肽、骨特异性碱性磷酸酶、E以及腓肠肌和比目鱼肌。
在老年ovx大鼠中观察到骨质疏松症、肌肉减少症和肥胖症的共现，同时伴有显著（P < 0.05）的脂肪量增加（30%）、骨质流失（9.6%）、归一化肌肉质量与体重比降低（10.5%）以及身体活动显著减少（57%）。在两个ovx年龄组中，胫骨骨矿物质密度与肌肉总质量的比值均显著降低。
卵巢切除大鼠可作为实验模型，用于在控制能量摄入和消耗的同时，研究卵巢激素丧失的影响，从而在不受到遗传背景混杂影响的情况下，开展女性肥胖和身体成分的转化研究。
