Liu Xiaoguang, Dai Chencheng, Jia Genmei, Xu Sujuan, Fu Ziyi, Xu Juan, Li Qing, Ruan Hongjie, Xu Pengfei
Nanjing Maternal and Child Health Institute, Nanjing Maternal and Child Health Care Hospital, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.
Department of Clinical Laboratory, Nanjing Maternal and Child Health Care Hospital, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.
Oncol Rep. 2017 Aug;38(2):799-808. doi: 10.3892/or.2017.5741. Epub 2017 Jun 22.
Recent studies suggest that long non-coding RNAs (lncRNAs) play crucial roles in many types of human malignant cancers. However, the function of lncRNAs in benign tumors remains poorly understood. In the present study, to explored the potential roles of lncRNAs in benign epithelial ovarian cysts (BEOCs) which commonly occur in young women and possess malignant potential, we described the expression profile of the lncRNAs between BEOC and normal ovarian tissues using lncRNA microarray techniques. The results showed that 1,325 transcripts of lncRNAs (1,014 upregulated and 311 downregulated) were differentially expressed in the BEOCs compared with the normal controls [absolute fold-change ≥2, false discovery rate (FDR) <0.05]. We also conducted quantitative real-time PCR (qPCR) to confirm the microarray data. The results of qPCR revealed that the expression trend of 6 randomly selected lncRNAs was consistent with the microarray data. Furthermore, candidate lncRNAs were characterized by pathway analysis and Gene Ontology (GO). The present study is the first to demonstrate different expression profiles of lncRNAs between BEOCs and normal ovarian tissues. These lncRNAs may play a crucial role in the pathological process of BEOCs.
近期研究表明，长链非编码RNA（lncRNAs）在多种人类恶性肿瘤中发挥着关键作用。然而，lncRNAs在良性肿瘤中的功能仍知之甚少。在本研究中，为探究lncRNAs在常见于年轻女性且具有恶性潜能的良性上皮性卵巢囊肿（BEOCs）中的潜在作用，我们使用lncRNA微阵列技术描述了BEOC与正常卵巢组织之间lncRNAs的表达谱。结果显示，与正常对照相比，BEOCs中有1325个lncRNA转录本（1014个上调和311个下调）差异表达[绝对倍数变化≥2，错误发现率（FDR）<0.05]。我们还进行了定量实时PCR（qPCR）以确认微阵列数据。qPCR结果显示，随机选择的6个lncRNAs的表达趋势与微阵列数据一致。此外，通过通路分析和基因本体论（GO）对候选lncRNAs进行了表征。本研究首次证明了BEOCs与正常卵巢组织之间lncRNAs的不同表达谱。这些lncRNAs可能在BEOCs的病理过程中发挥关键作用。
