Vernoux J P, Lahlou N, Abbad el Andaloussi S, Riyeche N, Magras L P
Acta Trop. 1985 Sep;42(3):225-33.
Ciguatera toxins were extracted from the tissues of 36 poisonous fishes including 9 dangerous species collected in the Caribbean. Toxicity assays were carried out in mice and the distinctive symptoms of ciguatera poisoning were observed. In a single fish, ciguatoxin was found in the blood, flesh, gonads, gills, heart, skin and bones. The concentration was highest in the viscera and in particular in the liver, kidney and spleen. The ratios of the toxin concentrations of the liver or viscera to that of the flesh were high and varied with the species suggesting that the toxin is stored in different ways in different fish. Subcellular fractionation of liver cells revealed that most of the ciguatoxin was attached to cytoplasmic proteins and that some toxin was probably bound to the membranes.
从加勒比地区采集的包括9种危险物种在内的36种有毒鱼类的组织中提取了雪卡毒素。在小鼠身上进行了毒性试验，并观察到雪卡中毒的明显症状。在一条鱼中，在血液、鱼肉、性腺、鳃、心脏、皮肤和骨骼中都发现了雪卡毒素。其浓度在内脏中最高，尤其是在肝脏、肾脏和脾脏中。肝脏或内脏与鱼肉中毒素浓度的比值很高，且因物种而异，这表明毒素在不同鱼类中的储存方式不同。肝细胞的亚细胞分级分离显示，大部分雪卡毒素附着在细胞质蛋白上，并且一些毒素可能与细胞膜结合。
