Deml E, Oesterle D
Cancer Lett. 1985 Oct;29(1):59-63. doi: 10.1016/0304-3835(85)90123-5.
Chloroform enhances dose-dependently the number of preneoplastic foci in livers of weanling female Sprague-Dawley rats. The preneoplastic foci were induced with a single dose of 8 mg diethylnitrosamine (DEN)/kg body wt. Thereafter chloroform was applied twice weekly for 11 consecutive weeks in doses of 100, 200 and 400 mg/kg body wt, respectively. This treatment raised the number of adenosine-5'-triphosphatase (ATPase)-deficient foci up to 5-fold, that of gamma-glutamyltranspeptidase (GGTase) and glycogen-positive foci 13- and 10-fold, respectively, after 12 weeks; 25 mg caused no effect compared to DEN-treated controls. In contrast, daily doses of chloroform only, 200 and 400 mg/kg body wt for 33 days, and 800 mg/kg body wt for 20 days given to 3-4-week-old female Sprague-Dawley rats did not lead to island formation, measured after 12 weeks, indicating a promoting rather than an initiating potency.
氯仿可剂量依赖性地增加断奶雌性斯普拉格-道利大鼠肝脏中癌前病灶的数量。癌前病灶通过单次给予8毫克/千克体重的二乙基亚硝胺（DEN）诱导产生。此后，氯仿分别以100、200和400毫克/千克体重的剂量每周两次连续应用11周。12周后，这种处理使腺苷-5'-三磷酸酶（ATPase）缺乏的病灶数量增加至5倍，γ-谷氨酰转肽酶（GGTase）和糖原阳性病灶数量分别增加13倍和10倍；与DEN处理的对照组相比，25毫克剂量没有效果。相比之下，对3-4周龄雌性斯普拉格-道利大鼠每日分别给予200和400毫克/千克体重的氯仿33天，以及800毫克/千克体重的氯仿20天，12周后测量未导致岛状形成，表明其具有促进作用而非启动作用。
