Zhou Lin, Wang Lu, Zhang Jialing, Li Jiahe, Bai Shuju, Ma Junfeng, Fu Xueqi
National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin Province, China.
PeerJ. 2019 Jan 9;6:e6218. doi: 10.7717/peerj.6218. eCollection 2019.
Didymin, a type of flavono-o-glycoside compound naturally present in citrus fruits, has been reported to be an effective anticancer agent. However, its effects on stress resistance are unclear. In this study, we treated with didymin at several concentrations. We found that didymin reduced the effects of UV stressor on nematodes by decreasing reactive oxygen species levels and increasing superoxide dismutase (SOD) activity. Furthermore, we found that specific didymin-treated mutant nematodes (mu86) & (e1370), (mu86), (ok525), (ok393), and (hx546) were susceptible to UV irradiation, whereas (e1371) was resistant to UV irradiation. In addition, we found that didymin not only promoted DAF-16 to transfer from cytoplasm to nucleus, but also increased both protein and mRNA expression levels of SOD-3 and HSP-16.2 after UV irradiation. Our results show that didymin affects UV irradiation resistance and it may act on to regulate downstream genes through the insulin/IGF-1-like signaling pathway.
二氢杨梅素是一种天然存在于柑橘类水果中的黄酮 - O - 糖苷化合物，据报道是一种有效的抗癌剂。然而，其对抗逆性的影响尚不清楚。在本研究中，我们用几种浓度的二氢杨梅素处理线虫。我们发现二氢杨梅素通过降低活性氧水平和增加超氧化物歧化酶（SOD）活性来减轻紫外线应激对线虫的影响。此外，我们发现经二氢杨梅素处理的特定突变线虫（mu86）、（e1370）、（mu86）、（ok525）、（ok393）和（hx546）对紫外线照射敏感，而（e1371）对紫外线照射具有抗性。另外，我们发现二氢杨梅素不仅促进DAF - 16从细胞质转移到细胞核，还在紫外线照射后增加了SOD - 3和HSP - 16.2的蛋白质和mRNA表达水平。我们的结果表明，二氢杨梅素影响紫外线照射抗性，并且它可能通过胰岛素/IGF - 1样信号通路作用于线虫以调节下游基因。
