da Silva Jessica H S, Simas Naomi K, Alviano Celuta S, Alviano Daniela S, Ventura José A, de Lima Eliandro J, Seabra Sergio H, Kuster Ricardo M
a Instituto de Pesquisas de Produtos Naturais, Bloco H, Centro de Ciências da Saúde , Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil.
b Faculdade de Farmácia, Centro de Ciências da Saúde , Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil.
Nat Prod Res. 2018 Jun;32(11):1365-1368. doi: 10.1080/14786419.2017.1344657. Epub 2017 Jul 3.
Ethanol extracts obtained from Schinus terebinthifolius Raddi fruits and leaves were active against Escherichia coli with MIC of 78 μg mL for both extracts. Phytochemical analyses revealed a major presence of phenolic acids, tannins, fatty acids and acid triterpenes in the leaves and phenolic acids, fatty acids, acid triterpenes and biflavonoids in the fruits. Major compounds isolated from the plant, such as the acid triterpene schinol, the phenolic acid derivative ethyl gallate and the biflavonoids agathisflavone and tetrahydroamentoflavone, showed very little activity against E. coli. Bioautography of the ethanol extracts on silica gel plate showed inhibition zones for E. coli. They were removed from the plate and the compounds identified as a mixture of myristic, pentadecanoic, palmitic, heptadecanoic, stearic, nonadecanoic, eicosanoic, heneicosanoic and behenic fatty acids.
从胡椒树果实和叶子中提取的乙醇提取物对大肠杆菌具有活性，两种提取物的最低抑菌浓度（MIC）均为78μg/mL。植物化学分析表明，叶子中主要含有酚酸、单宁、脂肪酸和三萜酸，果实中含有酚酸、脂肪酸、三萜酸和双黄酮。从该植物中分离出的主要化合物，如三萜酸schinol、酚酸衍生物没食子酸乙酯以及双黄酮阿盖黄酮和四氢穗花杉双黄酮，对大肠杆菌的活性非常低。乙醇提取物在硅胶板上的生物自显影显示出对大肠杆菌的抑制区。将这些区域从板上取下，鉴定出的化合物为肉豆蔻酸、十五烷酸、棕榈酸、十七烷酸、硬脂酸、十九烷酸、二十烷酸、二十一烷酸和山嵛酸的混合物。
