Bulla Milena K, Hernandes Luzmarina, Baesso Mauro L, Nogueira Ana C, Bento Antonio C, Bortoluzzi Bruno B, Serra Lara Z, Cortez Diogenes A G
Program of Post-Graduation in Pharmaceutical Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil.
Photochem Photobiol. 2015 May-Jun;91(3):558-66. doi: 10.1111/php.12419. Epub 2015 Feb 18.
Schinus terebinthifolius is a plant rich in phenolic compounds, which have antioxidant properties and can provide new opportunities for treatment and prevention of diseases mediated by ultraviolet radiation like photoaging and skin cancer. The aim of this study was to evaluate the photoprotective potential and ex vivo percutaneous penetration of the crude extract of Schinus terebinthifolius leaves. The extract was tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and β-carotene bleaching test. The sun protection factor was also evaluated. The ex vivo skin permeation of the emulsion and gel formulations were assayed. Fractionation of the extract resulted in gallic acid, ethyl gallate and a mixture of flavonoids, suggesting derivatives of quercetin and myricetin. The phenolic content of the extract was 384.64 ± 2.60 mg GAE g(-1) extract. The antioxidant activity was superior to butylated hydroxytoluene, in DPPH method, and ascorbic acid and rutin, in β-carotene bleaching assay. The extract showed UV absorption with photoprotector potential in the UVB region. The photoacoustic spectroscopy measurements confirmed absorption in the UV region and topical application of the formulations caused no histological changes in the rats' skin. These results suggest that the crude extract of Schinus terebinthifolius leaves may be a promising natural sunscreen product.
巴西胡椒是一种富含酚类化合物的植物，这些酚类化合物具有抗氧化特性，可为治疗和预防由紫外线辐射介导的疾病（如光老化和皮肤癌）提供新的机遇。本研究的目的是评估巴西胡椒叶粗提物的光保护潜力和离体经皮渗透情况。使用2,2-二苯基-1-苦基肼（DPPH）法和β-胡萝卜素漂白试验对提取物进行抗氧化活性测试。还评估了防晒系数。测定了乳液和凝胶制剂的离体皮肤渗透情况。提取物的分级分离得到了没食子酸、没食子酸乙酯和黄酮类混合物，提示为槲皮素和杨梅素的衍生物。提取物的酚含量为384.64±2.60mg GAE g(-1)提取物。在DPPH法中，其抗氧化活性优于丁基化羟基甲苯，在β-胡萝卜素漂白试验中优于抗坏血酸和芦丁。提取物在UVB区域显示出具有光保护潜力的紫外线吸收。光声光谱测量证实了在紫外线区域的吸收，并且制剂的局部应用未引起大鼠皮肤的组织学变化。这些结果表明，巴西胡椒叶粗提物可能是一种有前景的天然防晒产品。
