Departments of Dermatology and Ophthalmology, Institute for Regenerative Cures, University of California Davis, 2921 Stockton Boulevard, Sacramento, CA 95817, USA; Department of Neurosurgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, People's Republic of China; Shanghai Institute of Head Trauma, Shanghai 200127, People's Republic of China.
Stem Cell Program and Institute for Regenerative Cures, University of California Davis, Sacramento, CA 95817, USA.
Stem Cell Reports. 2017 Jul 11;9(1):177-189. doi: 10.1016/j.stemcr.2017.05.035. Epub 2017 Jun 29.
Limited migration of neural stem cells in adult brain is a roadblock for the use of stem cell therapies to treat brain diseases and injuries. Here, we report a strategy that mobilizes and guides migration of stem cells in the brain in vivo. We developed a safe stimulation paradigm to deliver directional currents in the brain. Tracking cells expressing GFP demonstrated electrical mobilization and guidance of migration of human neural stem cells, even against co-existing intrinsic cues in the rostral migration stream. Transplanted cells were observed at 3 weeks and 4 months after stimulation in areas guided by the stimulation currents, and with indications of differentiation. Electrical stimulation thus may provide a potential approach to facilitate brain stem cell therapies.
成年大脑中神经干细胞的迁移能力有限，这是将干细胞疗法用于治疗脑部疾病和损伤的一个障碍。在这里，我们报告了一种在体内动员和引导大脑干细胞迁移的策略。我们开发了一种安全的刺激范式，在大脑中传递定向电流。追踪表达 GFP 的细胞表明，即使在嗅球迁移流中存在共存的内在信号，人类神经干细胞也能被电动员和引导迁移。在刺激后的 3 周和 4 个月，在刺激电流引导的区域观察到移植细胞，并显示出分化的迹象。因此，电刺激可能为促进脑干细胞治疗提供一种潜在的方法。
