片上头颈肿瘤的维持：个性化治疗的途径？
Maintenance of head and neck tumor on-chip: gateway to personalized treatment?
作者信息
Bower Ruth, Green Victoria L, Kuvshinova Elena, Kuvshinov Dmitriy, Karsai Laszlo, Crank Stephen T, Stafford Nicholas D, Greenman John
机构信息
School of Life Sciences, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK.
Department of Chemical & Biological Engineering, The University of Sheffield, Mappin Street, Sheffield, S1 3JD, UK.
出版信息
Future Sci OA. 2017 Mar 7;3(2):FSO174. doi: 10.4155/fsoa-2016-0089. eCollection 2017 Jun.
AIM
Head and neck squamous cell carcinomas (HNSCC) are solid tumors with low overall survival (40-60%). In a move toward personalized medicine, maintenance of tumor biopsies in microfluidic tissue culture devices is being developed.
METHODOLOGY/RESULTS: HNSCC (n = 15) was dissected (5-10 mg) and either analyzed immediately or cultured in a microfluidic device (37°C) for 48 h. No difference was observed in morphology between pre- and postculture specimens. Dissociated samples were analyzed using trypan blue exclusion (viability), propidium iodide flow cytometry (death) and MTS assay (proliferation) with no significant difference observed highlighting tissue maintenance. Computational fluid dynamics showed laminar flow within the system.
CONCLUSION
The microfluidic culture system successfully maintained HNSCC for 48 h, the culture system will allow testing of different treatment modalities with response monitoring.
目的
头颈部鳞状细胞癌（HNSCC）是总体生存率较低（40%-60%）的实体瘤。朝着个性化医疗的方向发展，正在开发用于在微流控组织培养装置中维持肿瘤活检样本的方法。
方法/结果：解剖HNSCC样本（n = 15，5-10毫克），要么立即进行分析，要么在微流控装置中于37°C培养48小时。培养前后的样本在形态上未观察到差异。使用台盼蓝排斥法（活力）、碘化丙啶流式细胞术（死亡情况）和MTS分析（增殖情况）对解离后的样本进行分析未观察到显著差异，突出了组织维持效果。计算流体动力学显示系统内为层流。
结论
微流控培养系统成功维持HNSCC样本48小时，该培养系统将允许在监测反应的同时测试不同的治疗方式。
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