Jafarzadeh Abdollah, Arabi Zahra, Ahangar-Parvin Rayhaneh, Mohammadi-Kordkhayli Marziyeh, Nemati Maryam
Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Department of Immunology, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Drug Res (Stuttg). 2017 Nov;67(11):632-639. doi: 10.1055/s-0043-113455. Epub 2017 Jul 3.
Chemokines facilitate the leukocytes infiltration into the central nervous system (CNS) which is an essential step in the pathogenesis of multiple sclerosis. Ginger has also a broad anti-inflammatory properties. The aim was to evaluate the effects of ginger extract on the expression of CCL20 and CCL22 and their receptors (CCR6 and CCR4, respectively) in experimental autoimmune encephalomyelitis (EAE). Female C57BL/6 mice used for EAE induction by immunization with myelin oligodendroglial glycoprotein. Then, the EAE mice were treated with PBS or ginger extract, from day +3 to +30. At day 31, mice were scarified and the expression of CCL20 and CCL22 and their receptors in the spinal cord measured using real time-PCR. The expression of CCL20, CCL22 and CCR4 in the spinal cord of PBS-administrated EAE mice was significantly higher than healthy group (P<0.04, P<0.05 and P<0.02, respectively). In 200- and 300 mg/kg ginger extract-treated EAE mice, the expression of CCL20, CCL22 and CCR4 were significantly reduced as compared with PBS-administrated EAE group (P<0.04, P<0.01 and P<0.002 for 200 mg/kg ginger extract and P<0.01, P<0.005 and P<0.004 for 300 mg/kg ginger extract, respectively). The CCR6 expression in EAE mice treated with 200- or 300 mg/kg ginger extracts was lower than PBS-administrated EAE mice (P<0.01 and P=0.07, respectively). Treatment of EAE mice with ginger extract down-regulate the expression of CCL20 and CCL22 and their receptors in EAE mice. The possible therapeutic potential of ginger for treatment of MS can be considered in future investigations.
趋化因子促进白细胞浸润进入中枢神经系统（CNS），这是多发性硬化发病机制中的一个重要步骤。生姜也具有广泛的抗炎特性。本研究旨在评估生姜提取物对实验性自身免疫性脑脊髓炎（EAE）中CCL20和CCL22及其受体（分别为CCR6和CCR4）表达的影响。通过用髓鞘少突胶质细胞糖蛋白免疫来诱导雌性C57BL/6小鼠发生EAE。然后，从第3天到第30天，用PBS或生姜提取物对EAE小鼠进行治疗。在第31天，处死小鼠，使用实时PCR测量脊髓中CCL20和CCL22及其受体的表达。给予PBS的EAE小鼠脊髓中CCL20、CCL22和CCR4的表达显著高于健康组（分别为P<0.04、P<0.05和P<0.02）。与给予PBS的EAE组相比，用200和300mg/kg生姜提取物治疗的EAE小鼠中，CCL20、CCL22和CCR4的表达显著降低（200mg/kg生姜提取物组分别为P<0.04、P<0.01和P<0.002，300mg/kg生姜提取物组分别为P<0.01、P<0.005和P<0.004）。用200或300mg/kg生姜提取物治疗的EAE小鼠中CCR6的表达低于给予PBS的EAE小鼠（分别为P<0.01和P=0.07）。用生姜提取物治疗EAE小鼠可下调EAE小鼠中CCL20和CCL22及其受体的表达。在未来的研究中可以考虑生姜治疗MS的潜在治疗潜力。
