使用格里斯试剂法测定小鼠巨噬细胞中一氧化氮的优化孵育方案。
Optimized incubation regime for nitric oxide measurements in murine macrophages using the Griess assay.
作者信息
Schmölz Lisa, Wallert Maria, Lorkowski Stefan
机构信息
Department of Nutritional Biochemistry and Physiology, Institute of Nutrition, Friedrich Schiller University Jena, Dornburger Straße 25, 07743 Jena, Germany; Competence Cluster for Nutrition and Cardiovascular Health (nutriCARD), Halle-Jena-Leipzig, Germany.
Department of Nutritional Biochemistry and Physiology, Institute of Nutrition, Friedrich Schiller University Jena, Dornburger Straße 25, 07743 Jena, Germany; Baker Heart and Diabetes Institute, 75 Commercial Rd, Melbourne, VIC 3004, Australia.
出版信息
J Immunol Methods. 2017 Oct;449:68-70. doi: 10.1016/j.jim.2017.06.012. Epub 2017 Jul 1.
The Griess assay is used to measure nitric oxide concentrations in liquid solutions after reaction into nitrite. The assay is challenging when applied to cell culture supernatants. During optimization, we focused on the anti-inflammatory potential of test compounds in murine RAW264.7 macrophages. This led to (i) the required inductivity of cells by lipopolysaccharide (LPS) and allowed (ii) the characterization of putative anti-inflammatory test compounds with high sensitivity. The modifications reported here prominently improved resolution and efficiency of the widely used Griess assay and are of broad interest for studies on the pharmacological modulation of macrophages activation.
格里斯测定法用于测量反应生成亚硝酸盐后液体溶液中的一氧化氮浓度。该测定法应用于细胞培养上清液时具有挑战性。在优化过程中，我们关注了受试化合物在小鼠RAW264.7巨噬细胞中的抗炎潜力。这导致了（i）通过脂多糖（LPS）对细胞的所需诱导性，并允许（ii）以高灵敏度表征假定的抗炎受试化合物。此处报道的改进显著提高了广泛使用的格里斯测定法的分辨率和效率，对于巨噬细胞激活的药理调节研究具有广泛的意义。
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