Țicolea Mădălina, Pop Raluca Maria, Pârvu Marcel, Usatiuc Lia-Oxana, Uifălean Ana, Ranga Floricuța, Pârvu Alina Elena
Department of Morpho-Functional Sciences, Discipline of Pathophysiology, Iuliu Haţieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania.
Department of Morpho-Functional Sciences, Discipline of Pharmacology, Toxicology and Clinical Pharmacology, Iuliu Haţieganu University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania.
Antioxidants (Basel). 2024 Aug 20;13(8):1016. doi: 10.3390/antiox13081016.
This study aimed to investigate the antioxidant and anti-inflammatory activities mechanism of () and () ethanol extracts in acute rat inflammation induced in Wistar male rats with turpentine oil. The characterization of the polyphenolic compounds in the extracts was conducted using UV-Vis and Fourier-transform infrared spectroscopy and high-performance liquid chromatography coupled with mass spectrometry techniques. The antioxidant activity of the extracts was evaluated in vitro by DPPH, FRAP, HO, and NO scavenging tests and in vivo by measuring the total oxidative status (TOS), total antioxidant capacity (TAC), oxidative stress index (OSI), 8-hydroxy-deoxyguanosine (8-Oxo-dG), advanced oxidation protein products (AOPP), malondialdehyde (MDA), nitric oxide (NO), 3-nitrotyrosine (3NT), and total thiols (SH). Inflammation was evaluated by measuring nuclear factor-kB-p65 (NfkB-p65) and NLRP3 inflammasome activation with IL-1β, IL-18, and gasdermin D. Liver and renal toxicity was determined following transaminases (ALT and AST), creatinine, and urea. The experimental results indicated that and ethanol extracts have moderate in vitro antioxidant activity and had in vivo antioxidant activity and an anti-inflammatory effect by NfkB-p65, IL-1b, IL-18, and gasdermin D serum level reduction. The antioxidant activity correlated with the chemical composition of the extracts. These results bring evidence-based use of and in traditional and contemporary medicine.
本研究旨在探讨（）和（）乙醇提取物在松节油诱导的雄性Wistar大鼠急性炎症中的抗氧化和抗炎活性机制。采用紫外可见光谱、傅里叶变换红外光谱以及高效液相色谱-质谱联用技术对提取物中的多酚类化合物进行表征。通过DPPH、FRAP、HO和NO清除试验在体外评估提取物的抗氧化活性，并通过测量总氧化状态（TOS）、总抗氧化能力（TAC）、氧化应激指数（OSI）、8-羟基脱氧鸟苷（8-氧代-dG）、晚期氧化蛋白产物（AOPP）、丙二醛（MDA）、一氧化氮（NO）、3-硝基酪氨酸（3NT）和总硫醇（SH）在体内评估其抗氧化活性。通过测量核因子-κB-p65（NfkB-p65）以及NLRP3炎性小体与白细胞介素-1β（IL-1β）、白细胞介素-18（IL-18）和gasdermin D的激活来评估炎症。通过转氨酶（ALT和AST）、肌酐和尿素来测定肝脏和肾脏毒性。实验结果表明，（提取物名称）和（提取物名称）乙醇提取物具有中等程度的体外抗氧化活性，并且通过降低NfkB-p65、IL-1β、IL-18和gasdermin D的血清水平具有体内抗氧化活性和抗炎作用。抗氧化活性与提取物的化学成分相关。这些结果为（提取物名称）和（提取物名称）在传统医学和现代医学中的循证应用提供了依据。
原文括号处内容缺失，以上译文为尽量完整呈现结构的版本。
