Theodoro Letícia Helena, Ferro-Alves Marcio Luiz, Longo Mariéllen, Nuernberg Marta Aparecida Alberton, Ferreira Renata Pironato, Andreati Adriele, Ervolino Edilson, Duque Cristiane, Garcia Valdir Gouveia
São Paulo State University (Unesp), School of Dentistry, Araçatuba, Department of Surgery and Integrated Clinic, Division of Periodontics, Araçatuba, São Paulo, Brazil.
São Paulo State University (Unesp), School of Dentistry, Araçatuba, Department of Basic Science, Araçatuba, São Paulo, Brazil.
Lasers Med Sci. 2017 Nov;32(8):1783-1791. doi: 10.1007/s10103-017-2261-3. Epub 2017 Jul 3.
This study assessed the effect of curcumin as a photosensitizer in antimicrobial photodynamic therapy (aPDT) for the treatment of induced periodontitis in rats. Periodontitis was induced via a ligature around the mandibular first molar on the left side of 96 rats. The ligature was removed 7 days later, and the animals were randomized into four groups: NT, no local treatment; CUR, irrigation with curcumin solution (40 μM); LED, irradiation with a light-emitting diode (LED, InGaN, 465-485 nm, 200 mW/cm, 60 s); and aPDT, irrigation with curcumin solution (40 μM) followed by irradiation with LED. Eight animals from each group were euthanized at 7, 15, and 30 days post-treatment. Treatments were assessed using alveolar bone loss (ABL) in the furcation region using histological, histometric, and immunohistochemical analyses. Rats treated with aPDT exhibited less ABL at 7 days compared to the NT group, moderate pattern immunolabeling for osteoprotegerin at 30 days, and a pattern of immunolabeling for RANKL from moderate to low. Treatments resulted in smaller numbers of TRAP-positive cells compared to the NT group. aPDT as monotherapy using curcumin as a photosensitizer and LED as the light source was effective in the treatment of induced periodontitis in rats.
本研究评估了姜黄素作为光动力抗菌疗法（aPDT）中的光敏剂对大鼠实验性牙周炎的治疗效果。通过在96只大鼠左侧下颌第一磨牙周围结扎丝线诱导牙周炎。7天后移除结扎丝线，将动物随机分为四组：NT组，不进行局部治疗；CUR组，用姜黄素溶液（40μM）冲洗；LED组，用发光二极管照射（LED，InGaN，465 - 485nm，200mW/cm²，60秒）；aPDT组，先用姜黄素溶液（40μM）冲洗，然后用LED照射。每组8只动物在治疗后7天、15天和30天安乐死。使用组织学、组织计量学和免疫组织化学分析通过分叉区牙槽骨吸收（ABL）评估治疗效果。与NT组相比，aPDT组在7天时ABL较少，在30天时骨保护素呈中度模式免疫标记，核因子κB受体活化因子配体（RANKL）免疫标记模式从中度到低度。与NT组相比，治疗导致抗酒石酸酸性磷酸酶（TRAP）阳性细胞数量减少。以姜黄素作为光敏剂、LED作为光源的aPDT单一疗法对大鼠实验性牙周炎有效。
