Li Yu, Zhang Zhan-Zhao
Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People's Republic of China.
Department of Aesthetic Surgery, Hangzhou Raily Aesthetic Plastic Hospital, Hangzhou 310003, People's Republic of China.
Drug Des Devel Ther. 2018 May 24;12:1453-1466. doi: 10.2147/DDDT.S154334. eCollection 2018.
Excessive reactive oxygen species production caused by type 2 diabetes conditions can disrupt normal bone metabolism and greatly impair bone regeneration.
In the present study, curcumin (Cur)-loaded microspheres were incorporated into a fish collagen nano-hydroxyapatite scaffold to promote bone repair under diabetic conditions by inhibiting the reactive oxygen species production.
The drug release kinetic study showed that the Cur release from the composite scaffolds lasted up to 30 days. The sustained curcumin release from the scaffold significantly inhibited the overproduction of reactive oxygen species in mesenchymal stem cells caused by diabetic serum. Moreover, the Cur-loaded scaffold also remarkedly alleviated the negative effects of diabetic serum on the proliferation, migration, and osteogenic differentiation of mesenchymal stem cells. When implanted into bone defects in type 2 diabetic rats, the Cur-loaded scaffold also showed a greater bone formation capability compared to the pure scaffold.
The results of this study suggested that the novel controlled Cur release system may provide a promising route to improve bone regeneration in type 2 diabetic patients.
2型糖尿病状态导致的活性氧过量产生会扰乱正常的骨代谢并严重损害骨再生。
在本研究中，将载有姜黄素（Cur）的微球掺入鱼胶原蛋白纳米羟基磷灰石支架中，以通过抑制活性氧的产生来促进糖尿病条件下的骨修复。
药物释放动力学研究表明，复合支架中Cur的释放持续长达30天。支架中姜黄素的持续释放显著抑制了糖尿病血清引起的间充质干细胞中活性氧的过量产生。此外，载有Cur的支架还显著减轻了糖尿病血清对间充质干细胞增殖、迁移和成骨分化的负面影响。当植入2型糖尿病大鼠的骨缺损中时，与纯支架相比，载有Cur的支架也显示出更强的骨形成能力。
本研究结果表明，新型姜黄素控释系统可能为改善2型糖尿病患者的骨再生提供一条有前景的途径。
