Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, P. R. China.
Department of Hepatopancreatobiliary Surgery, the First Affiliated Hospital, Fujian Medical University, Fuzhou, 350005, P. R. China.
Sci Rep. 2017 Jul 4;7(1):4586. doi: 10.1038/s41598-017-04771-w.
Complete surgical resection is the only option for improving the survival of patients with ductal adenocarcinoma in the pancreatic head. After resection, determining the status of resection margins (RMs) is crucial for deciding on the nature of the follow-up treatment. The purpose of this study was to evaluate whether multiphoton microscopy (MPM) could be considered a reliable tool for determining the status of pancreatic neck margins by identifying tumour cells of ductal adenocarcinoma in these margins in the pancreatic head, and our results were affirmative. In particular, MPM could identify tumour cells in the nerves. It was also found that the quantification of the difference between normal duct cells and tumour cells was possible. In addition, the content of collagen could be quantified and used as a marker for differentiating ductal adenocarcinoma in the pancreatic head from normal pancreatic tissues, eventually leading to the identification of R0 and R1 resections of the pancreatic neck margin. With the development of the clinical applications of the multiphoton endoscope, MPM has the potential to provide in vivo real-time identification of RM status during surgery.
胰头导管腺癌患者的唯一生存选择是进行完全手术切除。切除后，确定切缘（RM）的状态对于决定后续治疗的性质至关重要。本研究旨在评估多光子显微镜（MPM）是否可以通过识别头颈部胰管腺癌肿瘤细胞来可靠地判断胰颈切缘状态，我们的结果是肯定的。特别是，MPM 可以识别神经中的肿瘤细胞。还发现，正常导管细胞与肿瘤细胞之间的差异定量是可能的。此外，胶原含量也可以定量，并用作区分头颈部胰管腺癌与正常胰腺组织的标志物，最终识别胰颈切缘的 R0 和 R1 切除。随着多光子内窥镜临床应用的发展，MPM 有可能在手术过程中提供 RM 状态的实时体内识别。
