中国甘肃白牦牛中 的检测。
Detection of in White Yaks in Gansu Province, China.
机构信息
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, China.
College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province 130118, China.
出版信息
Biomed Res Int. 2017;2017:5790181. doi: 10.1155/2017/5790181. Epub 2017 Jun 6.
, the most common zoonotic pathogen of microsporidiosis, has been found in various animals and humans, but no information is available concerning the prevalence and genotypes of in white yaks . In the present study, 353 faecal samples from white yaks in Tianzhu Tibetan Autonomous County, Gansu Province, Northwestern China, were collected and examined by PCR amplification of the internal transcribed spacer gene to estimate prevalence and identify their genotypes. Of the 353 faecal samples, 4 (1.13%) were tested -positive. Sequences analysis revealed that two known genotypes, namely, I ( = 1) and BEB4 ( = 2), and a novel genotype, namely, WCY1 ( = 1), were found in this study. Among them, genotype WCY1 was clustered into Group 1, and genotypes I and BEB4 belonged to Group 2. The present study firstly indicates the existence of in yaks in Gansu Province, Northwestern China. This is also the first record of in white yaks. Effective measures should be taken to control infection in white yaks, other animals, and humans.
微小隐孢子虫最常见的动物源性病原体，已在各种动物和人类中发现，但关于在白牦牛中的流行情况和基因型尚无信息。本研究采集了中国西北地区甘肃省天祝藏族自治县 353 份白牦牛粪便样本，通过内部转录间隔区基因的 PCR 扩增来检测微小隐孢子虫的流行情况并鉴定其基因型。在 353 份粪便样本中，有 4 份（1.13%）呈阳性。序列分析显示，本研究中发现了两种已知的基因型，即 I（=1）和 BEB4（=2），以及一种新的基因型，即 WCY1（=1）。其中，基因型 WCY1 聚类为第 1 组，基因型 I 和 BEB4 属于第 2 组。本研究首次表明在中国甘肃省牦牛中存在微小隐孢子虫。这也是在白牦牛中发现微小隐孢子虫的首例记录。应采取有效措施控制白牦牛、其他动物和人类的微小隐孢子虫感染。
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