利用无染色体整合系统从人脂肪组织来源的间充质干细胞中生成人诱导多能干细胞系KSCBi003-A。
Generation of a human induced pluripotent stem cell line, KSCBi003-A, from human adipose tissue-derived mesenchymal stem cells using a chromosomal integration-free system.
作者信息
Choi Hye Young, Kim So-Jung, Go Gue Youn, Kwon Ara, Im Young Sam, Ha Hye-Yeong, Hong Jin Tae, Jung Ji-Won, Koo Soo Kyung
机构信息
Division of Intractable Disease, National Center for Stem cell and Regenerative Medicine, Korea National Institute of Health, Chungcheongbuk-do, South Korea; College of Pharmacy, Chungbuk National University, Chungcheongbuk-do, South Korea.
Division of Intractable Disease, National Center for Stem cell and Regenerative Medicine, Korea National Institute of Health, Chungcheongbuk-do, South Korea.
出版信息
Stem Cell Res. 2018 Aug;31:1-4. doi: 10.1016/j.scr.2018.06.003. Epub 2018 Jun 27.
We generated a human induced pluripotent stem cell (hiPSC) line, KSCBi003-A, from adipose tissue-derived mesenchymal stem cells (Ad-MSCs) using a Sendai virus-based gene delivery system. We confirmed that the KSCBi003-A has a normal karyotype and short tandem repeat (STR)-based identities that match the parent cells. We also confirmed that the cell line expresses pluripotent stem cell markers such as Nanog, OCT4, SSEA-4, TRA-1-60, and TRA-1-81. We also analyzed that the KSCBi003-A has an ability to differentiate three germ layers (ectoderm, mesoderm, endoderm). This cell line is registered and available at the National Stem Cell Bank, Korea National Institute of Health.
我们使用基于仙台病毒的基因递送系统，从脂肪组织来源的间充质干细胞（Ad-MSCs）中生成了人诱导多能干细胞（hiPSC）系KSCBi003-A。我们证实KSCBi003-A具有正常的核型和与亲本细胞匹配的基于短串联重复序列（STR）的身份。我们还证实该细胞系表达多能干细胞标志物，如Nanog、OCT4、SSEA-4、TRA-1-60和TRA-1-81。我们还分析了KSCBi003-A具有分化为三个胚层（外胚层、中胚层、内胚层）的能力。该细胞系已在韩国国立卫生研究院国家干细胞库注册并可供使用。
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