Choi Hye Young, Kim So-Jung, Go Gue Youn, Kwon Ara, Im Young Sam, Ha Hye-Yeong, Hong Jin Tae, Jung Ji-Won, Koo Soo Kyung
Division of Intractable Disease, National Center for Stem cell and Regenerative Medicine, Korea National Institute of Health, Chungcheongbuk-do, South Korea; College of Pharmacy, Chungbuk National University, Chungcheongbuk-do, South Korea.
Division of Intractable Disease, National Center for Stem cell and Regenerative Medicine, Korea National Institute of Health, Chungcheongbuk-do, South Korea.
Stem Cell Res. 2018 Aug;31:1-4. doi: 10.1016/j.scr.2018.06.003. Epub 2018 Jun 27.
We generated a human induced pluripotent stem cell (hiPSC) line, KSCBi003-A, from adipose tissue-derived mesenchymal stem cells (Ad-MSCs) using a Sendai virus-based gene delivery system. We confirmed that the KSCBi003-A has a normal karyotype and short tandem repeat (STR)-based identities that match the parent cells. We also confirmed that the cell line expresses pluripotent stem cell markers such as Nanog, OCT4, SSEA-4, TRA-1-60, and TRA-1-81. We also analyzed that the KSCBi003-A has an ability to differentiate three germ layers (ectoderm, mesoderm, endoderm). This cell line is registered and available at the National Stem Cell Bank, Korea National Institute of Health.
我们使用基于仙台病毒的基因递送系统，从脂肪组织来源的间充质干细胞（Ad-MSCs）中生成了人诱导多能干细胞（hiPSC）系KSCBi003-A。我们证实KSCBi003-A具有正常的核型和与亲本细胞匹配的基于短串联重复序列（STR）的身份。我们还证实该细胞系表达多能干细胞标志物，如Nanog、OCT4、SSEA-4、TRA-1-60和TRA-1-81。我们还分析了KSCBi003-A具有分化为三个胚层（外胚层、中胚层、内胚层）的能力。该细胞系已在韩国国立卫生研究院国家干细胞库注册并可供使用。
