Ryu Ji Hyeon, Xie Chengliang, Kim Eun-Jin, Park Si-Hyang, Choi Yeung Joon, Kang Sang Soo, Shin Min-Kyoung, Kang Dawon
Department of Convergence Medical Science, Gyeongsang National University, Jinju 52727, Korea.
Department of Physiology and Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju 52727, Korea.
Nutrients. 2017 Jul 5;9(7):699. doi: 10.3390/nu9070699.
Sea hare has a variety of biological activities. However, little is known regarding the anti-asthmatic effects of sea hare. This study was performed to identify the effect of sea hare hydrolysates (SHH) on an ovalbumin (OVA)-induced allergic asthma model. The experimental asthma model was sensitized and challenged with OVA. We found that a high-dose of SHH (HSHH) significantly inhibited OVA-induced airway inflammation and mucus production around the airway in lung sections, while low- and medium-dose SHH showed an insignificant effect. In addition, HSHH highly reduced OVA-induced production of interleukin-4, -5, -13, leukotriene D4, E4, and histamine in bronchoalveolar lavage fluid. HSHH decreased the histamine-induced increase in the intracellular Ca level and contractions in asthmatic smooth muscle cells. Furthermore, HSHH did not affect the weights of the spleen nor thymus, whereas dexamethasone (DEX), a steroidal anti-inflammatory drug, reduced them. Taken together, these results showed that HSHH reduced asthmatic parameters in a mouse model of allergic asthma, and suggest that SHH could be used as a potential therapeutic agent for asthma.
海兔具有多种生物活性。然而，关于海兔的抗哮喘作用却知之甚少。本研究旨在确定海兔水解物（SHH）对卵清蛋白（OVA）诱导的过敏性哮喘模型的影响。实验性哮喘模型用OVA进行致敏和激发。我们发现高剂量的SHH（HSHH）能显著抑制OVA诱导的肺部切片气道周围的炎症和黏液分泌，而低剂量和中剂量的SHH则效果不明显。此外，HSHH能显著降低OVA诱导的支气管肺泡灌洗液中白细胞介素-4、-5、-13、白三烯D4、E4和组胺的产生。HSHH降低了组胺诱导的哮喘平滑肌细胞内钙离子水平的升高和收缩。此外，HSHH对脾脏和胸腺的重量没有影响，而甾体抗炎药地塞米松（DEX）则会使其重量减轻。综上所述，这些结果表明HSHH可降低过敏性哮喘小鼠模型中的哮喘参数，并提示SHH可作为哮喘的潜在治疗药物。
