CBMN, UMR5248, 33600 Pessac, France.
Nanoscale. 2017 Jul 13;9(27):9762-9769. doi: 10.1039/c7nr01901g.
Control of transport across membranes, whether natural or synthetic, is fundamental in many biotechnology applications, including sensing and drug release. Mutations of naturally existing protein channels, such as hemolysin, have been explored in the past. More recently, DNA channels with conductivities in the nanosiemens range have been designed. Regulating transport across DNA channels in response to external stimuli remains an important challenge. Previous designs relied on steric hindrance to control the inner diameter of the channel, which resulted in unstable electric signatures. In this paper we introduce a new design to control electric channel conductance of a DNA nanopore. The tensegrity driven mechanism inhibits the flux of small analytes while keeping a tightly controlled ionic transport modulated by the addition of specific DNA sequences. Current signals are clearly defined, with no sign of gating, opening new perspectives in single molecule DNA sensing.
控制跨膜运输，无论是天然的还是合成的，在许多生物技术应用中都是至关重要的，包括传感和药物释放。过去已经探索了自然存在的蛋白质通道（如溶血素）的突变。最近，设计了具有纳西门子范围内电导率的 DNA 通道。响应外部刺激调节 DNA 通道的运输仍然是一个重要的挑战。以前的设计依赖于空间位阻来控制通道的内径，这导致了不稳定的电信号。在本文中，我们引入了一种新的设计来控制 DNA 纳米孔的电导。张力驱动机制抑制了小分子分析物的通量，同时保持了由特定 DNA 序列的添加调制的紧密控制的离子传输。电流信号清晰定义，没有门控的迹象，为单分子 DNA 传感开辟了新的视角。
