HMGB3通过调节WNT/β-连环蛋白信号通路促进结直肠癌的生长和迁移。
HMGB3 promotes growth and migration in colorectal cancer by regulating WNT/β-catenin pathway.
作者信息
Zhang Zheying, Chang Yaya, Zhang Jianming, Lu Yanxia, Zheng Lin, Hu Yuhan, Zhang Fan, Li Xiaomin, Zhang Wenjuan, Li Xuenong
机构信息
Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
Department of Pathology, Xinxiang Medical University, Xinxiang, Henan, China.
出版信息
PLoS One. 2017 Jul 5;12(7):e0179741. doi: 10.1371/journal.pone.0179741. eCollection 2017.
Colorectal cancer (CRC) is the third leading cause of cancer-related deaths and a major health problem. High mobility group box 3 (HMGB3), a member of the high-mobility group box (HMGB) family, was reported to be over-expressed in gastric carcinoma and bladder cancer. However, the function of HMGB3 in CRC remains unclear. Here, we found that HMGB3 was up-regulated in CRC at both mRNA and protein levels. qRT-PCR results showed that high expression of HMGB3 had positive correlation with serosal invasion, lymph metastasis, and tumor-node-metastasis (TNM) stage in CRC patient. Functional experiments showed that HMGB3 can promote CRC cells proliferation and migration in vitro. Moreover, we found HMGB3 can active WNT/β-catenin pathway to increase the expression level of c-Myc and MMP7. These results may be the reason for HMGB3 oncogene role in CRC. In summary, our data indicated that HMGB3 may serve as an oncoprotein and could be used as a potential prognostic marker in CRC.
结直肠癌（CRC）是癌症相关死亡的第三大主要原因，也是一个重大的健康问题。高迁移率族蛋白盒3（HMGB3）是高迁移率族蛋白盒（HMGB）家族的成员之一，据报道在胃癌和膀胱癌中过度表达。然而，HMGB3在结直肠癌中的功能仍不清楚。在此，我们发现HMGB3在结直肠癌中的mRNA和蛋白质水平均上调。qRT-PCR结果显示，HMGB3的高表达与结直肠癌患者的浆膜侵犯、淋巴结转移及肿瘤-淋巴结-转移（TNM）分期呈正相关。功能实验表明，HMGB3可促进结直肠癌细胞在体外的增殖和迁移。此外，我们发现HMGB3可激活WNT/β-连环蛋白通路，以增加c-Myc和MMP7的表达水平。这些结果可能是HMGB3在结直肠癌中发挥癌基因作用的原因。总之，我们的数据表明，HMGB3可能作为一种癌蛋白，并可作为结直肠癌潜在的预后标志物。
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