State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai 200237, PR China.
Bioresour Technol. 2017 Dec;245(Pt B):1641-1644. doi: 10.1016/j.biortech.2017.06.105. Epub 2017 Jun 23.
In this study, stepwise increases in linalool production were obtained by combining metabolic engineering and process optimization of an unconventional oleaginous yeast Yarrowia lipolytica. The linalool synthetic pathway was successfully constructed by heterologously expressing a codon-optimized linalool synthase gene from Actinidia arguta in Y. lipolytica. To enhance linalool productivity, key genes involved in the mevalonate pathway were overexpressed in different combinations. Moreover, the overexpression of mutant ERG20 gene resulted in further linalool production. A maximum linalool titre of 6.96±0.29mg/L was achieved in shake flasks, which was the highest level ever reported in yeasts.
在这项研究中，通过组合代谢工程和非常规油脂酵母解脂耶氏酵母的过程优化，获得了芳樟醇产量的逐步增加。通过在解脂耶氏酵母中异源表达猕猴桃来源的经过密码子优化的芳樟醇合酶基因，成功构建了芳樟醇合成途径。为了提高芳樟醇的生产力，以不同组合的方式过表达了参与甲羟戊酸途径的关键基因。此外，过表达突变的 ERG20 基因导致进一步的芳樟醇生产。在摇瓶中获得了 6.96±0.29mg/L 的最高芳樟醇产量，这是酵母中报道的最高水平。
