Laboratoire de Chimie Analytique Bio-inorganique et Environnement (LCABIE), UMR 5254-IPREM, CNRS-UPPA, Hélioparc, Pau, France.
Metallomics. 2017 Aug 16;9(8):1014-1027. doi: 10.1039/c7mt00054e.
The use of nickel in modern industry and in consumer products implies some health problems for the human being. Nickel allergy and nickel carcinogenicity are well-known health effects related to human exposure to nickel, either during production of nickel-containing products or by direct contact with the final item. In this context, the study of nickel toxicity and nickel carcinogenicity involves the understanding of their molecular mechanisms and hence the characterization of the nickel-binding proteins in different biological samples. During the last 50 years, a broad range of analytical techniques, covering from the first chromatographic columns to the last generation mass spectrometers, have been used in order to fully characterize the nickel proteome. The aim of this review is to present a critical view of the different analytical approaches that have been applied for the purification, isolation, detection and identification of nickel-binding proteins. The different analytical techniques used are discussed from a critical point of view, highlighting advantages and limitations.
镍在现代工业和消费产品中的使用给人类健康带来了一些问题。镍过敏和镍致癌性是与人类接触镍有关的众所周知的健康影响，无论是在生产含镍产品期间还是通过直接接触最终产品。在这种情况下，研究镍毒性和镍致癌性涉及对其分子机制的理解，从而对不同生物样本中的镍结合蛋白进行特征描述。在过去的 50 年中，为了全面描述镍蛋白质组，已经使用了从第一代色谱柱到最新一代质谱仪的广泛的分析技术。本综述的目的是批判性地介绍已应用于纯化、分离、检测和鉴定镍结合蛋白的不同分析方法。从批判性的角度讨论了不同的分析技术，突出了它们的优点和局限性。
