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大鼠脑发育过程中两种神经细胞特异性β-微管蛋白mRNA的差异表达。

Differential expression of two neural cell-specific beta-tubulin mRNAs during rat brain development.

作者信息

Bond J F, Robinson G S, Farmer S R

出版信息

Mol Cell Biol. 1984 Jul;4(7):1313-9. doi: 10.1128/mcb.4.7.1313-1319.1984.

DOI:10.1128/mcb.4.7.1313-1319.1984
PMID:6095061
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368913/
Abstract

We recently demonstrated that beta-tubulin mRNA expression is regulated during rat brain development. This is manifested by a dramatic decrease in both 1.8- and 2.9-kilobase (kb) mRNAs when extensive neurite elongation is occurring. Coincident with these decreases is the increased production of a 2.5-kb mRNA. (J.F. Bond and S.R. Farmer, Mol. Cell. Biol. 3:1333-1342, 1983). In the present study, we have isolated and characterized three different cDNAs corresponding to beta-tubulin mRNAs (R beta T.1, R beta T.2, and R beta T.3). Hybridization of 3' untranslated region subclones of R beta T.1 and R beta T.2 cDNAs to RNA from a variety of rat tissues and cells revealed that these two cDNAs are neural cell specific. R beta T.1 corresponds to an abundant 1.8-kb mRNA expressed only at early stages of rat brain development. R beta T.2 corresponds to the 2.5-kb mRNA expressed at later stages. These data strongly suggest that there is differential expression of the beta-tubulin multigene family during rat brain development.

摘要

我们最近证明，β-微管蛋白mRNA的表达在大鼠脑发育过程中受到调控。这表现为在广泛的神经突伸长发生时，1.8千碱基（kb）和2.9千碱基的mRNA均显著减少。与这些减少同时发生的是2.5千碱基mRNA产量的增加。（J.F.邦德和S.R.法默，《分子与细胞生物学》3:1333 - 1342，1983年）。在本研究中，我们分离并鉴定了三种与β-微管蛋白mRNA相对应的不同cDNA（RβT.1、RβT.2和RβT.3）。RβT.1和RβT.2 cDNA的3'非翻译区亚克隆与来自多种大鼠组织和细胞的RNA杂交显示，这两种cDNA是神经细胞特异性的。RβT.1对应于仅在大鼠脑发育早期表达的丰富的1.8千碱基mRNA。RβT.2对应于在后期表达的2.5千碱基mRNA。这些数据强烈表明，在大鼠脑发育过程中β-微管蛋白多基因家族存在差异表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/368913/395e95c432c3/molcellb00149-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/368913/9c15d3ab5fa7/molcellb00149-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/368913/6a7c2ed5edfb/molcellb00149-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/368913/d2c806e5f203/molcellb00149-0132-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/368913/2ad052790a89/molcellb00149-0132-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/368913/395e95c432c3/molcellb00149-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/368913/9c15d3ab5fa7/molcellb00149-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/368913/6a7c2ed5edfb/molcellb00149-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/368913/d2c806e5f203/molcellb00149-0132-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/368913/2ad052790a89/molcellb00149-0132-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/368913/395e95c432c3/molcellb00149-0133-a.jpg

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